119646 20250908131402.0 doi 10.1002/vms3.572 sideral 125835 ART-2021-125835 eng Rodríguez-Largo A. Universidad de Zaragoza (orcid)0000-0002-2685-1730 Detection of aluminium hydroxide-induced granulomas in sheep by computed tomography: A feasible approach for small ruminant lentiviruses diagnosis and research 2021 Access copy available to the general public Unrestricted Aluminium (Al) hydroxide use as adjuvant induces local formation of long-lasting subcutaneous granulomas in sheep. Macrophages within these granulomas have been identified as a new small ruminant lentivirus (SRLV) replication site in naturally infected animals. Diagnosis of Al hydroxide-induced granulomas in sheep is mostly based on postmortem observations but little information is available on in vivo detection. Computed tomography (CT) is used for studying these reactions in other animal species. To determine if CT could be a tool for in vivo diagnosis and research of subcutaneous Al hydroxide-induced granulomas in sheep. A retrospective survey on thoracic CT scans was performed on 46 adult sheep. Analysis included absence or presence, number and location of subcutaneous nodules. Thoracic CT scans and pathological studies were prescribed to two further sheep. Single or multiple subcutaneous nodules were detected in 26 (56.52%) sheep. One or two nodules per animal were most often observed (36.95%). Size ranged between 1.5 and 4.5 cm. Pre-contrast two-dimensional (2D) CT images showed focal or multifocal increases in subcutaneous tissue thickness. Post-contrast 2D CT images revealed hypointense areas in the centre. Histopathology indicated the presence of granulomas composed by a large number of activated macrophages, surrounding a central core of necrosis. Large intracytoplasmic Al-positive aggregates were demonstrated by lumogallion staining. CT is a useful tool to detect subcutaneous Al hydroxide-induced granulomas in vivo in sheep. CT provides a diagnostic and research tool that can be very useful in future works in Al hydroxide-induced pathology, SRLV infection, or both. © 2021 The Authors. Veterinary Medicine and Science published by John Wiley & Sons Ltd. info:eu-repo/grantAgreement/ES/DGA/A17-17R info:eu-repo/grantAgreement/ES/MICINN/RTI2018-096172-B-C33 info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 1.776 2021 VETERINARY SCIENCES 61 / 145 = 0.421 2021 Q2 T2 0.427 2021 Veterinary (miscellaneous) 2021 Q2 1.8 2021 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Castells E. de Miguel R. Gómez Á. (orcid)0000-0002-9723-9004 Ruiz H. Universidad de Zaragoza (orcid)0000-0002-8474-2831 Pérez M. Universidad de Zaragoza (orcid)0000-0002-8133-2124 Reina R. Ferrer L.M. Universidad de Zaragoza (orcid)0000-0003-0042-8800 Luján L. Universidad de Zaragoza (orcid)0000-0002-2053-9842 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 1001 025 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Anatom.Anatom.Patológ.Com 1009 617 Universidad de Zaragoza Dpto. Patología Animal Área Medicina y Cirugía Animal 7, 5 (2021), 1757-1761 Vet. med. sci. Veterinary medicine and science 2053-1095 3067905 http://zaguan.unizar.es/record/119646/files/texto_completo.pdf Versión publicada 2094553 http://zaguan.unizar.es/record/119646/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:119646 articulos driver 2025-09-08-12:48:48 ARTICLE