000119651 001__ 119651
000119651 005__ 20230519145457.0
000119651 0247_ $$2doi$$a10.1186/s13071-021-04841-z
000119651 0248_ $$2sideral$$a126126
000119651 037__ $$aART-2021-126126
000119651 041__ $$aeng
000119651 100__ $$aAguilar-Vega C.
000119651 245__ $$aA study of the composition of the Obsoletus complex and genetic diversity of Culicoides obsoletus populations in Spain
000119651 260__ $$c2021
000119651 5060_ $$aAccess copy available to the general public$$fUnrestricted
000119651 5203_ $$aBackground: The Culicoides obsoletus species complex (henceforth ‘Obsoletus complex’) is implicated in the transmission of several arboviruses that can cause severe disease in livestock, such as bluetongue, African horse sickness, epizootic hemorrhagic disease and Schmallenberg disease. Thus, this study aimed to increase our knowledge of the composition and genetic diversity of the Obsoletus complex by partial sequencing of the cytochrome c oxidase I (cox1) gene in poorly studied areas of Spain. Methods: A study of C. obsoletus populations was carried out using a single-tube multiplex polymerase chain reaction (PCR) assay that was designed to differentiate the Obsoletus complex sibling species Culicoides obsoletus and Culicoides scoticus, based on the partial amplification of the cox1 gene, as well as cox1 georeferenced sequences from Spain available at GenBank. We sampled 117 insects of the Obsoletus complex from six locations and used a total of 238 sequences of C. obsoletus (ss) individuals (sampled here, and from GenBank) from 14 sites in mainland Spain, the Balearic Islands and the Canary Islands for genetic diversity and phylogenetic analyses. Results: We identified 90 C. obsoletus (ss), 19 Culicoides scoticus and five Culicoides montanus midges from the six collection sites sampled, and found that the genetic diversity of C. obsoletus (ss) were higher in mainland Spain than in the Canary Islands. The multiplex PCR had limitations in terms of specificity, and no cryptic species within the Obsoletus complex were identified. Conclusions: Within the Obsoletus complex, C. obsoletus (ss) was the predominant species in the analyzed sites of mainland Spain. Information about the species composition of the Obsoletus complex could be of relevance for future epidemiological studies when specific aspects of the vector competence and capacity of each species have been identified. Our results indicate that the intraspecific divergence is higher in C. obsoletus (ss) northern populations, and demonstrate the isolation of C. obsoletus (ss) populations of the Canary Islands. Graphical abstract: [Figure not available: see fulltext.] © 2021, The Author(s).
000119651 536__ $$9info:eu-repo/grantAgreement/EC/H2020/727393/EU/Understanding pathogen, livestock, environment interactions involving bluetongue virus/PALE-Blu$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 727393-PALE-Blu
000119651 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000119651 590__ $$a4.052$$b2021
000119651 591__ $$aTROPICAL MEDICINE$$b3 / 24 = 0.125$$c2021$$dQ1$$eT1
000119651 591__ $$aPARASITOLOGY$$b8 / 39 = 0.205$$c2021$$dQ1$$eT1
000119651 592__ $$a1.089$$b2021
000119651 593__ $$aParasitology$$c2021$$dQ1
000119651 593__ $$aInfectious Diseases$$c2021$$dQ1
000119651 594__ $$a6.4$$b2021
000119651 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000119651 700__ $$aRivera B.
000119651 700__ $$0(orcid)0000-0003-0663-8411$$aLucientes J.$$uUniversidad de Zaragoza
000119651 700__ $$aGutiérrez-Boada I.
000119651 700__ $$aSánchez-Vizcaíno J.M.
000119651 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000119651 773__ $$g14, 1 (2021), 351 [13 pp]$$pParasites & Vectors$$tParasites and Vectors$$x1756-3305
000119651 8564_ $$s2972627$$uhttps://zaguan.unizar.es/record/119651/files/texto_completo.pdf$$yVersión publicada
000119651 8564_ $$s2423695$$uhttps://zaguan.unizar.es/record/119651/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000119651 909CO $$ooai:zaguan.unizar.es:119651$$particulos$$pdriver
000119651 951__ $$a2023-05-18-14:51:45
000119651 980__ $$aARTICLE