000119680 001__ 119680
000119680 005__ 20230519145620.0
000119680 0247_ $$2doi$$a10.3389/fimmu.2021.704621
000119680 0248_ $$2sideral$$a126245
000119680 037__ $$aART-2021-126245
000119680 041__ $$aeng
000119680 100__ $$aMateos-Hernández L.
000119680 245__ $$aAnti-microbiota vaccines modulate the tick microbiome in a taxon-specific manner
000119680 260__ $$c2021
000119680 5060_ $$aAccess copy available to the general public$$fUnrestricted
000119680 5203_ $$aThe lack of tools for the precise manipulation of the tick microbiome is currently a major limitation to achieve mechanistic insights into the tick microbiome. Anti-tick microbiota vaccines targeting keystone bacteria of the tick microbiota alter tick feeding, but their impact on the taxonomic and functional profiles of the tick microbiome has not been tested. In this study, we immunized a vertebrate host model (Mus musculus) with live bacteria vaccines targeting keystone (i.e., Escherichia-Shigella) or non-keystone (i.e., Leuconostoc) taxa of tick microbiota and tested the impact of bacterial-specific antibodies (Abs) on the structure and function of tick microbiota. We also investigated the effect of these anti-microbiota vaccines on mice gut microbiota composition. Our results showed that the tick microbiota of ticks fed on Escherichia coli-immunized mice had reduced Escherichia-Shigella abundance and lower species diversity compared to ticks fed on control mice immunized with a mock vaccine. Immunization against keystone bacteria restructured the hierarchy of nodes in co-occurrence networks and reduced the resistance of the bacterial network to taxa removal. High levels of E. coli-specific IgM and IgG were negatively correlated with the abundance of Escherichia-Shigella in tick microbiota. These effects were not observed when Leuconostoc was targeted with vaccination against Leuconostoc mesenteroides. Prediction of functional pathways in the tick microbiome using PICRUSt2 revealed that E. coli vaccination reduced the abundance of lysine degradation pathway in tick microbiome, a result validated by qPCR. In contrast, the gut microbiome of immunized mice showed no significant alterations in the diversity, composition and abundance of bacterial taxa. Our results demonstrated that anti-tick microbiota vaccines are a safe, specific and an easy-to-use tool for manipulation of vector microbiome. These results guide interventions for the control of tick infestations and pathogen infection/transmission. © Copyright © 2021 Mateos-Hernández, Obregón, Wu-Chuang, Maye, Bornères, Versillé, de la Fuente, Díaz-Sánchez, Bermúdez-Humarán, Torres-Maravilla, Estrada-Peña, Hodžic, Šimo and Cabezas-Cruz.
000119680 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000119680 590__ $$a8.787$$b2021
000119680 591__ $$aIMMUNOLOGY$$b35 / 163 = 0.215$$c2021$$dQ1$$eT1
000119680 592__ $$a2.331$$b2021
000119680 593__ $$aImmunology and Allergy$$c2021$$dQ1
000119680 593__ $$aImmunology$$c2021$$dQ1
000119680 594__ $$a9.8$$b2021
000119680 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000119680 700__ $$aObregón D.
000119680 700__ $$aWu-Chuang A.
000119680 700__ $$aMaye J.
000119680 700__ $$aBornères J.
000119680 700__ $$aVersillé N.
000119680 700__ $$ade la Fuente J.
000119680 700__ $$aDíaz-Sánchez S.
000119680 700__ $$aBermúdez-Humarán L.G.
000119680 700__ $$aTorres-Maravilla E.
000119680 700__ $$0(orcid)0000-0001-7483-046X$$aEstrada-Peña A.$$uUniversidad de Zaragoza
000119680 700__ $$aHodžic A.
000119680 700__ $$aŠimo L.
000119680 700__ $$aCabezas-Cruz A.
000119680 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000119680 773__ $$g12 (2021), 704621 [15 pp]$$pFront. immunol.$$tFrontiers in Immunology$$x1664-3224
000119680 8564_ $$s7293343$$uhttps://zaguan.unizar.es/record/119680/files/texto_completo.pdf$$yVersión publicada
000119680 8564_ $$s2567262$$uhttps://zaguan.unizar.es/record/119680/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000119680 909CO $$ooai:zaguan.unizar.es:119680$$particulos$$pdriver
000119680 951__ $$a2023-05-18-16:14:51
000119680 980__ $$aARTICLE