119729 20250319143308.0 doi 10.3390/foods11203282 sideral 130551 ART-2022-130551 eng Berdejo, Daniel Universidad de Zaragoza (orcid)0000-0001-5053-8309 Carvacrol selective pressure allows the occurrence of genetic resistant variants of Listeria monocytogenes EGD-e 2022 Access copy available to the general public Unrestricted Essential oils and their constituents, such as carvacrol, are potential food preservatives because of their great antimicrobial properties. However, the long-term effects of these compounds are unknown and raise the question of whether resistance to these antimicrobials could emerge. This work aims to evaluate the occurrence of genetic resistant variants (RVs) in Listeria monocytogenes EGD-e by exposure to carvacrol. Two protocols were performed for the RVs selection: (a) by continuous exposure to sublethal doses, where LmSCar was isolated, and (b) by reiterative exposure to short lethal treatments of carvacrol, where LmLCar was isolated. Both RVs showed an increase in carvacrol resistance. Moreover, LmLCar revealed an increased cross-resistance to heat treatments at acid conditions and to ampicillin. Whole-genome sequencing identified two single nucleotide variations in LmSCar and three non-silent mutations in LmLCar. Among them, those located in the genes encoding the transcriptional regulators RsbT (in LmSCar) and ManR (in LmLCar) could contribute to their increased carvacrol resistance. These results provide information regarding the mode of action of this antimicrobial and support the importance of knowing how RVs appear. Further studies are required to determine the emergence of RVs in food matrices and their impact on food safety. info:eu-repo/grantAgreement/ES/FEDER/Una manera de hacer Europa info:eu-repo/grantAgreement/ES/MICINN/PGC2018-093789-B-I00/AEI/10.13039/501100011033 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 5.2 2022 FOOD SCIENCE & TECHNOLOGY 34 / 142 = 0.239 2022 Q1 T1 0.771 2022 Food Science 2022 Q1 Health Professions (miscellaneous) 2022 Q1 Plant Science 2022 Q1 Microbiology 2022 Q2 Health (social science) 2022 Q2 5.8 2022 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Gayán, Elisa Universidad de Zaragoza (orcid)0000-0002-5895-2157 Pagán, Elisa Universidad de Zaragoza (orcid)0000-0003-3752-4660 Merino, Natalia Universidad de Zaragoza (orcid)0000-0003-4774-0973 Campillo, Raúl Universidad de Zaragoza (orcid)0000-0003-2579-5375 Pagán, Rafael Universidad de Zaragoza (orcid)0000-0002-0238-6328 García-Gonzalo, Diego Universidad de Zaragoza (orcid)0000-0002-7629-8101 2008 640 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Nutrición Bromatología 2008 780 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Tecnología de Alimentos 11, 20 (2022), 3282 [15 pp.] Foods Foods 2304-8158 1012741 http://zaguan.unizar.es/record/119729/files/texto_completo.pdf Versión publicada 2746719 http://zaguan.unizar.es/record/119729/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:119729 articulos driver 2025-03-19-14:32:18 ARTICLE