000119791 001__ 119791
000119791 005__ 20240319080957.0
000119791 0247_ $$2doi$$a10.1016/j.actatropica.2022.106371
000119791 0248_ $$2sideral$$a128281
000119791 037__ $$aART-2022-128281
000119791 041__ $$aeng
000119791 100__ $$0(orcid)0000-0001-6209-4282$$aVillanueva-Saz, Sergio$$uUniversidad de Zaragoza
000119791 245__ $$aEvaluation of five different rapid immunochromatographic tests for canine leishmaniosis in Spain
000119791 260__ $$c2022
000119791 5060_ $$aAccess copy available to the general public$$fUnrestricted
000119791 5203_ $$aCanine leishmaniosis is a vector-borne disease caused by Leishmania parasites. Serological methods are the most common tests used for the diagnosis. This study aimed to evaluate and compare different serological commercial immunochromatographic rapid tests available in Spain to detect anti-Leishmania canine antibodies. The immunochromatographic tests were evaluated in different groups of dogs (healthy seronegative dogs (n = 21), naturally-sick dogs with moderate anti-Leishmania antibodies (n = 39), naturally-sick dogs with high anti-Leishmania antibodies (n = 37), dogs with the serological result of other pathogens infection (n = 20) and exposed dogs (n = 33)) admitted to the Veterinary Teaching Hospital of the University of Zaragoza (Spain) according to the clinical information sent with the sample to the laboratory for diagnostic purposes. The serology status was also routinely recorded through an in-house enzyme-linked immunosorbent assay (ELISA) and an in-house indirect immunofluorescence test (IFAT). The qualitative commercial serological immunochromatographic tests used were: FASTest LEISH, Uranotest Leishmania, Uranotest Leishmania 2.0, Speed Leish K, Witness Leishmania, and DFV Test Leishmania. Performance measures analyzed for each test were: sensitivity, specificity, and area under the receiver-operating (ROC) curve. The maximum specificity (1.00) was attained for Uranotest Leishmania and DFT Test Leishmania, followed by FASTest LEISH (0.98), Uranotest Leishmania 2.0 (0.98), Speed Leish K (0.98), and Witness Leishmania (0.95). The maximum sensitivity was attained for FASTest LEISH (1.00), followed by Uranotest 2.0 (0.97), Speed Leish K (0.97), Uranotest (0.96), and the lowest results with Witness (0.84) and DFV Test (0.59). Regarding the ROC curve, the maximum value was attained with the FASTest LEISH (0.99), followed by Uranotest (0.98), Uranotest 2.0 (0.97), Speed Leish K (0.97), Witness (0.90), and the lowest result with DFV Test (0.79). Efforts in the field of diagnosis should focus on establishing a commercial immunochromatographic test with high sensitivity and specificity with a reasonable cost-benefit balance.
000119791 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000119791 590__ $$a2.7$$b2022
000119791 592__ $$a0.751$$b2022
000119791 591__ $$aTROPICAL MEDICINE$$b11 / 24 = 0.458$$c2022$$dQ2$$eT2
000119791 593__ $$aInsect Science$$c2022$$dQ1
000119791 591__ $$aPARASITOLOGY$$b15 / 37 = 0.405$$c2022$$dQ2$$eT2
000119791 593__ $$aVeterinary (miscellaneous)$$c2022$$dQ1
000119791 593__ $$aParasitology$$c2022$$dQ2
000119791 593__ $$aInfectious Diseases$$c2022$$dQ2
000119791 594__ $$a5.4$$b2022
000119791 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000119791 700__ $$aMartínez, Marivi
000119791 700__ $$aRamirez, Juan David
000119791 700__ $$aHerrera, Giovanny
000119791 700__ $$aMarteles, Diana
000119791 700__ $$aServián, Marina
000119791 700__ $$0(orcid)0000-0003-2957-1379$$aVerde, Maite$$uUniversidad de Zaragoza
000119791 700__ $$aGiner, Jacobo
000119791 700__ $$0(orcid)0000-0002-7822-6646$$aLacasta, Delia$$uUniversidad de Zaragoza
000119791 700__ $$0(orcid)0000-0002-8474-2831$$aRuíz, Héctor$$uUniversidad de Zaragoza
000119791 700__ $$aYzuel, Andrés
000119791 700__ $$0(orcid)0000-0002-2557-4890$$aFernández, Antonio$$uUniversidad de Zaragoza
000119791 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000119791 773__ $$g229 (2022), 106371 [5 pp]$$pActa Trop.$$tACTA TROPICA$$x0001-706X
000119791 8564_ $$s765888$$uhttps://zaguan.unizar.es/record/119791/files/texto_completo.pdf$$yVersión publicada
000119791 8564_ $$s2471694$$uhttps://zaguan.unizar.es/record/119791/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000119791 909CO $$ooai:zaguan.unizar.es:119791$$particulos$$pdriver
000119791 951__ $$a2024-03-18-13:44:13
000119791 980__ $$aARTICLE