119799 20240319081002.0 doi 10.1021/acssuschemeng.2c04016 sideral 130758 ART-2022-130758 eng Delso, Carlota Universidad de Zaragoza (orcid)0000-0003-4744-8649 Post-incubation pH Impacts the Lipid Extraction Assisted by Pulsed Electric Fields from Wet Biomass of "Auxenochlorella protothecoides" 2022 Access copy available to the general public Unrestricted Pulsed electric field (PEF) treatment is a promising technology for efficient lipid extraction from microalgae. This study focusses on under-investigated processing parameters, such as media pH, pulse application, and incubation protocols. The lipid yield and electroporation level of PEF-treated Auxenochlorella protothecoides were determined at a medium pH of 3.0 and 5.0 under variation of the pre- or post-PEF incubation time and for split-dose treatments. Low energetic PEF treatments with 40 kV/cm and 1 μs pulses at 9.6 and 19.2 kJ/L were performed either in batch mode or in continuous flow. Post-PEF incubation significantly increased the shared electroporated cells (>60%) in medium pH 3.0, while no change was observed at pH 5.0. Split-dose PEF treatments at pH 5.0 caused significantly higher electroporation levels and lipid extraction yields than equivalent single-dose treatments. Results have shown that medium pH is critical in the final electroporation and lipid extraction yields of A. protothecoides and therefore should be considered in further studies. info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 8.4 2022 ENGINEERING, CHEMICAL 13 / 141 = 0.092 2022 Q1 T1 CHEMISTRY, MULTIDISCIPLINARY 32 / 178 = 0.18 2022 Q1 T1 GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY 14 / 46 = 0.304 2022 Q2 T1 1.744 2022 Chemical Engineering (miscellaneous) 2022 Q1 Renewable Energy, Sustainability and the Environment 2022 Q1 Environmental Chemistry 2022 Q1 Chemistry (miscellaneous) 2022 Q1 15.3 2022 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Silve, Aude Wüstner, Rüdiger Nazarova, Natalja Álvarez, Ignacio Universidad de Zaragoza (orcid)0000-0003-2430-858X Raso, Javier Universidad de Zaragoza (orcid)0000-0003-3957-9091 Frey, Wolfgang 2008 780 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Tecnología de Alimentos 10, 37 (2022), 12448-12456 ACS sustain. chem. & eng. ACS Sustainable Chemistry and Engineering 2168-0485 1788985 http://zaguan.unizar.es/record/119799/files/texto_completo.pdf Versión publicada 3105651 http://zaguan.unizar.es/record/119799/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:119799 articulos driver 2024-03-18-14:15:54 ARTICLE