119830 20240319081006.0 doi 10.3390/nu14091986 sideral 129159 ART-2022-129159 eng Reigada, Inés Alterations in bacterial metabolism contribute to the Lifespan Extension Exerted by Guarana in Caenorhabditis elegans 2022 Access copy available to the general public Unrestricted Guarana (Paullinia cupana) is a widely consumed nutraceutical with various health benefits supported by scientific evidence. However, its indirect health impacts through the gut microbi-ota have not been studied. Caenorhabditis elegans is a useful model to study both the direct and indirect effects of nutraceuticals, as the intimate association of the worm with the metabolites produced by Escherichia coli is a prototypic simplified model of our gut microbiota. We prepared an ethanoic extract of guarana seeds and assessed its antioxidant capacity in vitro, with a 2, 2-diphenyl-1-pic-rylhydrazyl (DPPH) assay, and in vivo, utilizing C. elegans. Additionally, we studied the impact of this extract on C. elegans lifespan, utilizing both viable and non-viable E. coli, and assessed the impact of guarana on E. coli folate production. The extract showed high antioxidant capacity, and it extended worm lifespan. However, the antioxidant and life-extending effects did not correlate in terms of the extract concentration. The extract-induced life extension was also less significant when utilizing dead E. coli, which may indicate that the effects of guarana on the worms work partly through modifications on E. coli metabolism. Following this observation, guarana was found to decrease E. coli folate production, revealing one possible route for its beneficial effects. © 2022 by the authors. Licensee MDPI, Basel, Switzerland. info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 5.9 2022 NUTRITION & DIETETICS 17 / 87 = 0.195 2022 Q1 T1 1.291 2022 Nutrition and Dietetics 2022 Q1 Food Science 2022 Q1 9.0 2022 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Kapp, Karmen Maynard, Claire Weinkove, David Valero, Marta Sofía Universidad de Zaragoza (orcid)0000-0002-2231-7565 Langa, Elisa Hanski, Leena Gómez-Rincón, Carlota 1012 410 Universidad de Zaragoza Dpto. Farmac.Fisiol.y Med.L.F. Área Fisiología 14, 9 (2022), 1986 [15 pp.] Nutrients Nutrients 2072-6643 1360491 http://zaguan.unizar.es/record/119830/files/texto_completo.pdf Versión publicada 2761207 http://zaguan.unizar.es/record/119830/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:119830 articulos driver 2024-03-18-14:36:15 ARTICLE