000119904 001__ 119904
000119904 005__ 20240319081016.0
000119904 0247_ $$2doi$$a10.3389/fmicb.2022.956602
000119904 0248_ $$2sideral$$a130626
000119904 037__ $$aART-2022-130626
000119904 041__ $$aeng
000119904 100__ $$aAlebouyeh, Sogol
000119904 245__ $$aIron deprivation enhances transcriptional responses to in vitro growth arrest of Mycobacterium tuberculosis
000119904 260__ $$c2022
000119904 5060_ $$aAccess copy available to the general public$$fUnrestricted
000119904 5203_ $$aThe establishment of Mycobacterium tuberculosis (Mtb) long-term infection in vivo depends on several factors, one of which is the availability of key nutrients such as iron (Fe). The relation between Fe deprivation inside and outside the granuloma, and the capacity of Mtb to accumulate lipids and persist in the absence of growth is not well understood. In this context, current knowledge of how Mtb modifies its lipid composition in response to growth arrest, depending on iron availability, is scarce. To shed light on these matters, in this work we compare genome-wide transcriptomic and lipidomic profiles of Mtb at exponential and stationary growth phases using cultures with glycerol as a carbon source, in the presence or absence of iron. As a result, we found that transcriptomic responses to growth arrest, considered as the transition from exponential to stationary phase, are iron dependent for as many as 714 genes (iron-growth interaction contrast, FDR <0.05), and that, in a majority of these genes, iron deprivation enhances the magnitude of the transcriptional responses to growth arrest in either direction. On the one hand, genes whose upregulation upon growth arrest is enhanced by iron deprivation were enriched in functional terms related to homeostasis of ion metals, and responses to several stressful cues considered cardinal features of the intracellular environment. On the other hand, genes showing negative responses to growth arrest that are stronger in iron-poor medium were enriched in energy production processes (TCA cycle, NADH dehydrogenation and cellular respiration), and key controllers of ribosomal activity shut-down, such as the T/A system mazE6/F6. Despite of these findings, a main component of the cell envelope, lipid phthiocerol dimycocerosate (PDIM), was not detected in the stationary phase regardless of iron availability, suggesting that lipid changes during Mtb adaptation to non-dividing phenotypes appear to be iron-independent. Taken together, our results indicate that environmental iron levels act as a key modulator of the intensity of the transcriptional adaptations that take place in the bacterium upon its transition between dividing and dormant-like phenotypes in vitro.
000119904 536__ $$9info:eu-repo/grantAgreement/ES/MICINN/RYC-2017-23560$$9info:eu-repo/grantAgreement/ES/MICINN/PID2019-110240RB-I00$$9info:eu-repo/grantAgreement/ES/MICINN/PID2019-106859GA-I00$$9info:eu-repo/grantAgreement/ES/FEDER/PI19-00666$$9info:eu-repo/grantAgreement/ES/DGA/E36-20R
000119904 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000119904 590__ $$a5.2$$b2022
000119904 592__ $$a1.19$$b2022
000119904 591__ $$aMICROBIOLOGY$$b38 / 135 = 0.281$$c2022$$dQ2$$eT1
000119904 593__ $$aMicrobiology (medical)$$c2022$$dQ1
000119904 593__ $$aMicrobiology$$c2022$$dQ1
000119904 594__ $$a7.8$$b2022
000119904 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000119904 700__ $$0(orcid)0000-0003-0790-9367$$aCárdenas Pestana, Jorge A.$$uUniversidad de Zaragoza
000119904 700__ $$aVázquez, Lucia
000119904 700__ $$aPrados Rosales, Rafael
000119904 700__ $$aDel Portillo, Patricia
000119904 700__ $$0(orcid)0000-0002-2980-9685$$aSanz, Joaquín$$uUniversidad de Zaragoza
000119904 700__ $$aMenéndez, Maria Carmen
000119904 700__ $$aGarcía, Maria J.
000119904 7102_ $$12004$$2405$$aUniversidad de Zaragoza$$bDpto. Física Teórica$$cÁrea Física Teórica
000119904 773__ $$g13 (2022), [16 pp.]$$pFront. microbiol.$$tFrontiers in Microbiology$$x1664-302X
000119904 8564_ $$s4054125$$uhttps://zaguan.unizar.es/record/119904/files/texto_completo.pdf$$yVersión publicada
000119904 8564_ $$s2353618$$uhttps://zaguan.unizar.es/record/119904/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000119904 909CO $$ooai:zaguan.unizar.es:119904$$particulos$$pdriver
000119904 951__ $$a2024-03-18-15:38:43
000119904 980__ $$aARTICLE