000119911 001__ 119911
000119911 005__ 20240319081016.0
000119911 0247_ $$2doi$$a10.5424/sjar/2022203-19272
000119911 0248_ $$2sideral$$a130643
000119911 037__ $$aART-2022-130643
000119911 041__ $$aeng
000119911 100__ $$0(orcid)0000-0001-8932-4615$$aAmanzougarene, Zahia
000119911 245__ $$aFreezing preservation procedure of caecal inoculum for microbial fermentation studies in pigs
000119911 260__ $$c2022
000119911 5060_ $$aAccess copy available to the general public$$fUnrestricted
000119911 5203_ $$aAim of study: To define freezing conditions that preserve fermentative capacity of microbial inoculum for in vitro studies in pigs.
Material and methods: Caecal contents from three slaughtered pigs were obtained for being used as inoculum. Part of it was immediately frozen in liquid N and stored at -80ºC, whereas the rest was directly used as fermentation inocula. Incubation substrate was pre-digested in pepsin and pancreatin to simulate the processes occurring before the caecum. Pre-digested substrate was incubated alone or supplemented by three additives consisting of two commercial additives based on essential oils mixtures (CRINA-TEP and CRINA-TMEC) and riboflavin. Gas production at 2, 4, 6, 8, 10, and 12 h, and methane, short chain fatty acids (SCFA), and ammonia concentration at 6 h and 12 h were recorded.&#x0D.
000119911 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000119911 590__ $$a0.9$$b2022
000119911 592__ $$a0.249$$b2022
000119911 591__ $$aAGRICULTURE, MULTIDISCIPLINARY$$b40 / 58 = 0.69$$c2022$$dQ3$$eT3
000119911 593__ $$aAgronomy and Crop Science$$c2022$$dQ3
000119911 594__ $$a1.9$$b2022
000119911 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000119911 700__ $$aPérez-Calvo, Estefanía
000119911 700__ $$0(orcid)0000-0002-0712-1185$$aFondevila, Manuel$$uUniversidad de Zaragoza
000119911 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Producción Animal
000119911 773__ $$g20, 3 (2022), 7$$pSpan. j. agric. res.$$tSpanish Journal of Agricultural Research$$x1695-971X
000119911 8564_ $$s441941$$uhttps://zaguan.unizar.es/record/119911/files/texto_completo.pdf$$yVersión publicada
000119911 8564_ $$s2478240$$uhttps://zaguan.unizar.es/record/119911/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000119911 909CO $$ooai:zaguan.unizar.es:119911$$particulos$$pdriver
000119911 951__ $$a2024-03-18-15:39:54
000119911 980__ $$aARTICLE