doi:10.5424/sjar/2022203-19272engAmanzougarene, ZahiaPérez-Calvo, EstefaníaFondevila, ManuelFreezing preservation procedure of caecal inoculum for microbial fermentation studies in pigsART-2022-130643Aim of study: To define freezing conditions that preserve fermentative capacity of microbial inoculum for in vitro studies in pigs.
 Material and methods: Caecal contents from three slaughtered pigs were obtained for being used as inoculum. Part of it was immediately frozen in liquid N and stored at -80ºC, whereas the rest was directly used as fermentation inocula. Incubation substrate was pre-digested in pepsin and pancreatin to simulate the processes occurring before the caecum. Pre-digested substrate was incubated alone or supplemented by three additives consisting of two commercial additives based on essential oils mixtures (CRINA-TEP and CRINA-TMEC) and riboflavin. Gas production at 2, 4, 6, 8, 10, and 12 h, and methane, short chain fatty acids (SCFA), and ammonia concentration at 6 h and 12 h were recorded.&#x0D.2022http://zaguan.unizar.es/record/11991110.5424/sjar/2022203-19272http://zaguan.unizar.es/record/119911oai:zaguan.unizar.es:119911Spanish Journal of Agricultural Research 20, 3 (2022), 7byhttp://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccess