119911 20240319081016.0 doi 10.5424/sjar/2022203-19272 sideral 130643 ART-2022-130643 eng Amanzougarene, Zahia (orcid)0000-0001-8932-4615 Freezing preservation procedure of caecal inoculum for microbial fermentation studies in pigs 2022 Access copy available to the general public Unrestricted Aim of study: To define freezing conditions that preserve fermentative capacity of microbial inoculum for in vitro studies in pigs.
 Material and methods: Caecal contents from three slaughtered pigs were obtained for being used as inoculum. Part of it was immediately frozen in liquid N and stored at -80ºC, whereas the rest was directly used as fermentation inocula. Incubation substrate was pre-digested in pepsin and pancreatin to simulate the processes occurring before the caecum. Pre-digested substrate was incubated alone or supplemented by three additives consisting of two commercial additives based on essential oils mixtures (CRINA-TEP and CRINA-TMEC) and riboflavin. Gas production at 2, 4, 6, 8, 10, and 12 h, and methane, short chain fatty acids (SCFA), and ammonia concentration at 6 h and 12 h were recorded.&#x0D. info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 0.9 2022 AGRICULTURE, MULTIDISCIPLINARY 40 / 58 = 0.69 2022 Q3 T3 0.249 2022 Agronomy and Crop Science 2022 Q3 1.9 2022 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pérez-Calvo, Estefanía Fondevila, Manuel Universidad de Zaragoza (orcid)0000-0002-0712-1185 2008 700 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Producción Animal 20, 3 (2022), 7 Span. j. agric. res. Spanish Journal of Agricultural Research 1695-971X 441941 http://zaguan.unizar.es/record/119911/files/texto_completo.pdf Versión publicada 2478240 http://zaguan.unizar.es/record/119911/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:119911 articulos driver 2024-03-18-15:39:54 ARTICLE