119920 20240319081017.0 doi 10.1093/mmy/myac070 sideral 130713 ART-2022-130713 eng Dashti, Alejandro Zoonotic "Enterocytozoon bieneusi" genotypes in free-ranging and farmed wild ungulates in Spain 2022 Access copy available to the general public Unrestricted Microsporidia comprises a diverse group of obligate, intracellular, and spore-forming parasites that infect a wide range of animals. Among them, Enterocytozoon bieneusi is the most frequently reported species in humans and other mammals and birds. Data on the epidemiology of E. bieneusi in wildlife are limited. Hence, E. bieneusi was investigated in eight wild ungulate species present in Spain (genera Ammotragus, Capra, Capreolus, Cervus, Dama, Ovis, Rupicapra, and Sus) by molecular methods. Faecal samples were collected from free-ranging (n = 1058) and farmed (n = 324) wild ungulates from five Spanish bioregions. The parasite was detected only in red deer (10.4%, 68/653) and wild boar (0.8%, 3/359). Enterocytozoon bieneusi infections were more common in farmed (19.4%, 63/324) than in wild (1.5%, 5/329) red deer. A total of 11 genotypes were identified in red deer, eight known (BEB6, BEB17, EbCar2, HLJD-V, MWC_d1, S5, Type IV, and Wildboar3) and three novel (DeerSpEb1, DeerSpEb2, and DeerSpEb3) genotypes. Mixed genotype infections were detected in 15.9% of farmed red deer. Two genotypes were identified in wild boar, a known (Wildboar3) and a novel (WildboarSpEb1) genotypes. All genotypes identified belonged to E. bieneusi zoonotic Groups 1 and 2. This study provides the most comprehensive epidemiological study of E. bieneusi in Spanish ungulates to date, representing the first evidence of the parasite in wild red deer populations worldwide. Spanish wild boars and red deer are reservoir of zoonotic genotypes of E. bieneusi and might play an underestimated role in the transmission of this microsporidian species to humans and other animals info:eu-repo/grantAgreement/ES/MINECO/CGL2016-80543-P info:eu-repo/grantAgreement/ES/ISCIII-MINECO/PI19CIII/00029 info:eu-repo/grantAgreement/ES/MINECO/CGL2012-40043-C02-01 info:eu-repo/grantAgreement/ES/MINECO/CGL2012-40043-C02-02 info:eu-repo/grantAgreement/ES/MINECO/PID2020-115046GB-I00 info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 2.9 2022 VETERINARY SCIENCES 14 / 144 = 0.097 2022 Q1 T1 INFECTIOUS DISEASES 67 / 96 = 0.698 2022 Q3 T3 MYCOLOGY 16 / 30 = 0.533 2022 Q3 T2 0.742 2022 Veterinary (miscellaneous) 2022 Q1 Infectious Diseases 2022 Q2 Medicine (miscellaneous) 2022 Q2 7.3 2022 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Santín, Mónica Köster, Pamela C Bailo, Begoña Ortega, Sheila Imaña, Elena Habela, Miguel Ángel Rivero-Juarez, Antonio Vicente, Joaquin Conejero, Carles González-Crespo, Carlos Garrido, Cristina Gassó, Diana Andrea Murillo, Diana Serrano, Emmanuel Mentaberre, Gregorio Torres-Blas, Irene Estruch, Josep Pastor, Josep Ramón López-Olvera, Jorge Escobar-González, María Valldeperes, Marta Mesalles, Montse López, Omar Álvarez, Raquel Cuenca, Rafaela Velarde, Roser Lavín, Santiago Arnal, Maria C Universidad de Zaragoza (orcid)0000-0002-6770-3400 Fernánez de Luco, Daniel Universidad de Zaragoza (orcid)0000-0003-3289-4267 Morrondo, Patrocinio Armenteros, José A Balseiro, Ana Cardona, Guillermo A Martínez-Carrasco, Carlos Ortiz, José Antonio Calero-Bernal, Rafael Carmena, David González-Barrio, David 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 60, 9 (2022), 070 [11 pp.] Med. mycol. MEDICAL MYCOLOGY 1369-3786 941477 http://zaguan.unizar.es/record/119920/files/texto_completo.pdf Versión publicada 3315514 http://zaguan.unizar.es/record/119920/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:119920 articulos driver 2024-03-18-15:45:22 ARTICLE