000119930 001__ 119930 000119930 005__ 20240319081018.0 000119930 0247_ $$2doi$$a10.1038/s41598-022-21013-w 000119930 0248_ $$2sideral$$a130769 000119930 037__ $$aART-2022-130769 000119930 041__ $$aeng 000119930 100__ $$aOwji, Nazanin 000119930 245__ $$aInclusion of calcium phosphate does not further improve in vitro and in vivo osteogenesis in a novel, highly biocompatible, mechanically stable and 3D printable polymer 000119930 260__ $$c2022 000119930 5060_ $$aAccess copy available to the general public$$fUnrestricted 000119930 5203_ $$aAt a time of unpredictable challenges for health, one trend is certain: there is an exceedingly high demand for functional implants, particularly bone grafts. This has encouraged the emergence of bone tissue engineering substitutes as an alternative method to conventional bone grafts. However, the current approaches in the field face several limitations that have prevented the ultimate translation into clinical settings. As a result, many attempts have been made to fabricate synthetic bone implants that can offer suitable biological and mechanical properties.Light curable methacrylate-based polymers have ideal properties for bone repair. These materials are also suitable for 3D printing which can be applicable for restoration of both function and aesthetics. The main objective of this research was to investigate the role of calcium phosphate (CaP) incorporation in a mechanically stable, biologically functional and 3D printable polymer for the reconstruction of complex craniofacial defects. The experimental work initially involved the synthesis of (((((((((((3R,3aR,6S,6aR)- hexahydrofuro[3,2-b]furan-3,6-diyl)bis(oxy))bis(ethane-2,1- 48 diyl))bis(oxy))bis(carbonyl))bis(azanediyl))bis(3,3,5-trimethylcyclohexane-5,1- 49 diyl))bis(azanediyl))bis(carbonyl))bis(oxy))bis(ethane-2,1-diyl) bis(2-methylacrylate) referred to as CSMA and fabrication of composite discs via a Digital Light Printing (DLP) method. The flow behaviour of the polymer as a function of CaP addition, surface remineralisation potential, in vitro cell culture, using MC3T3 and Adipose-Derived Mesenchymal Stem Cells (ADSCs) and ex ovo angiogenic response was assessed. Finally, in vivo studies were carried out to investigate neo-bone formation at 4- and 8-weeks post-implantation. Quantitative micro-CT and histological evaluation did not show a higher rate of bone formation in CaP filled CSMA composites compared to CSMA itself. Therefore, such polymeric systems hold promising features by allowing more flexibility in designing a 3D printed scaffold targeted at the reconstruction of maxillofacial defects. 000119930 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000119930 590__ $$a4.6$$b2022 000119930 592__ $$a0.973$$b2022 000119930 591__ $$aMULTIDISCIPLINARY SCIENCES$$b22 / 73 = 0.301$$c2022$$dQ2$$eT1 000119930 593__ $$aMultidisciplinary$$c2022$$dQ1 000119930 594__ $$a7.5$$b2022 000119930 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000119930 700__ $$aMandakhbayar, Nandin 000119930 700__ $$aCha, Jae-Ryung 000119930 700__ $$aPadalhin, Andrew R. 000119930 700__ $$aErdogan, Zalike Keskin 000119930 700__ $$aAldaadaa, Alaa 000119930 700__ $$aShakouri, Taleen 000119930 700__ $$aSawadkar, Prasad 000119930 700__ $$aFrost, Oliver 000119930 700__ $$aKim, Hae-Won 000119930 700__ $$0(orcid)0000-0001-7062-9099$$aGarcía Gareta, Elena$$uUniversidad de Zaragoza 000119930 700__ $$aKnowles, Jonathan C. 000119930 7102_ $$15001$$2065$$aUniversidad de Zaragoza$$bDpto. Ciencia Tecnol.Mater.Fl.$$cÁrea Cienc.Mater. Ingen.Metal. 000119930 773__ $$g12 (2022), 16977 [16 pp]$$pSci. rep. (Nat. Publ. Group)$$tScientific reports (Nature Publishing Group)$$x2045-2322 000119930 8564_ $$s10601035$$uhttps://zaguan.unizar.es/record/119930/files/texto_completo.pdf$$yVersión publicada 000119930 8564_ $$s2366317$$uhttps://zaguan.unizar.es/record/119930/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000119930 909CO $$ooai:zaguan.unizar.es:119930$$particulos$$pdriver 000119930 951__ $$a2024-03-18-15:52:54 000119930 980__ $$aARTICLE