119939 20240319081018.0 doi 10.3390/molecules27175631 sideral 130813 ART-2022-130813 eng Goranov, B. Biopreservation of chocolate mousse with Lactobacillus helveticus 2/20: Microbial Challenge Test 2022 Access copy available to the general public Unrestricted Probiotic bacteria are used for food biopreservation because their metabolic products might contribute to ensuring food microbiological safety and/or increase its shelf life without the addition of chemical preservatives. Moreover, biopreserved foods are excellent vehicles for the delivery of probiotic bacteria. The aim of the study was to investigate the potential of chocolate mousse food matrix for the delivery of the probiotic strain Lactobacillus helveticus 2/20 (Lb. helveticus 2/20) and to investigate its capacity to inhibit the growth of two foodborne pathogenic bacteria (Staphylococcus aureus and Escherichia coli). Therefore, the populations of free or encapsulated in calcium alginate Lb. helveticus 2/20 cells and/or of each pathogen (used to voluntarily contaminate each sample) were monitored both in complex nutrient medium (MRS broth) and in chocolate mousse under refrigeration conditions and at room temperature. Lb. helveticus 2/20 alone in free or encapsulated state effectively inhibited the growth of Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923 in chocolate mousse when stored at 20 ± 2 °C. Practically no viable unwanted bacteria were identified on the 7th day from the beginning of the process. High viable Lb. helveticus 2/20 cell populations were maintained during storage under refrigerated conditions (4 ± 2 °C) and at room temperature. Chocolate mousse is thus a promising food matrix to deliver probiotic Lb. helveticus 2/20 cells, which could also protect it from contamination by unwanted bacteria. info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 4.6 2022 CHEMISTRY, MULTIDISCIPLINARY 63 / 178 = 0.354 2022 Q2 T2 BIOCHEMISTRY & MOLECULAR BIOLOGY 97 / 285 = 0.34 2022 Q2 T2 0.704 2022 Pharmaceutical Science 2022 Q1 Chemistry (miscellaneous) 2022 Q2 Drug Discovery 2022 Q2 Physical and Theoretical Chemistry 2022 Q2 Organic Chemistry 2022 Q2 Analytical Chemistry 2022 Q2 Medicine (miscellaneous) 2022 Q2 Molecular Medicine 2022 Q3 6.7 2022 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Teneva, D. Denkova-Kostova, R. Shopska, V. Oulahal, N. Denkova, Z. Kostov, G. Degraeve, P. Pagan, R. Universidad de Zaragoza (orcid)0000-0002-0238-6328 2008 780 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Tecnología de Alimentos 27, 17 (2022), 5631[24 pp.] Molecules (Basel, Online) Molecules 1420-3049 16157466 http://zaguan.unizar.es/record/119939/files/texto_completo.pdf Versión publicada 2745139 http://zaguan.unizar.es/record/119939/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:119939 articulos driver 2024-03-18-15:54:04 ARTICLE