000119999 001__ 119999
000119999 005__ 20240319080946.0
000119999 0247_ $$2doi$$a10.3390/ijms232113554
000119999 0248_ $$2sideral$$a130930
000119999 037__ $$aART-2022-130930
000119999 041__ $$aeng
000119999 100__ $$0(orcid)0000-0002-7277-4318$$aMoreno-Martinez, Laura$$uUniversidad de Zaragoza
000119999 245__ $$aHemizygous Granzyme A Mice Expressing the hSOD1G93A Transgene Show Slightly Extended Lifespan
000119999 260__ $$c2022
000119999 5060_ $$aAccess copy available to the general public$$fUnrestricted
000119999 5203_ $$aGranzyme A (gzmA), a serine protease involved in the modulation of the inflammatory immune response, is found at an elevated level in the serum from ALS patients. However, the influence of gzmA on the progression of ALS remains unclear. The aim of our work was to assess whether the absence of gzmA in an ALS murine model could help slow down the progression of the disease. Homozygous and hemizygous gzmA-deficient mice expressing the hSOD1G93A transgene were generated, and survival of these mice was monitored. Subsequently, gene and protein expression of inflammatory and oxidative stress markers was measured in the spinal cord and quadriceps of these mice. We observed the longest lifespan in gzmA+/− mice. GzmA gene and protein expression was downregulated in the spinal cord and serum from gmzA+/− mice, confirming that the increased survival of hemizygous mice is correlated with lower levels of gzmA. In addition, mRNA and protein levels of glutathione reductase (GSR), involved in oxidative stress, were found downregulated in the spinal cord and quadriceps of gmzA+/− mice, together with lower IL-1β and IL-6 mRNA levels in hemyzigous mice. In summary, our findings indicate for the first time that reduced levels, but not the absence, of gzmA could slightly ameliorate the disease progression in this animal model.
000119999 536__ $$9info:eu-repo/grantAgreement/ES/AEI/PID2020-113963RB-I00$$9info:eu-repo/grantAgreement/ES/DGA/B29-20R$$9info:eu-repo/grantAgreement/ES/ISCIII/FEDER/PI21-00372
000119999 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000119999 590__ $$a5.6$$b2022
000119999 592__ $$a1.154$$b2022
000119999 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b66 / 285 = 0.232$$c2022$$dQ1$$eT1
000119999 593__ $$aMedicine (miscellaneous)$$c2022$$dQ1
000119999 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b52 / 178 = 0.292$$c2022$$dQ2$$eT1
000119999 593__ $$aPhysical and Theoretical Chemistry$$c2022$$dQ1
000119999 593__ $$aComputer Science Applications$$c2022$$dQ1
000119999 593__ $$aInorganic Chemistry$$c2022$$dQ1
000119999 593__ $$aSpectroscopy$$c2022$$dQ1
000119999 593__ $$aOrganic Chemistry$$c2022$$dQ1
000119999 593__ $$aMolecular Biology$$c2022$$dQ2
000119999 593__ $$aCatalysis$$c2022$$dQ2
000119999 594__ $$a7.8$$b2022
000119999 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000119999 700__ $$aSantiago, Llipsy
000119999 700__ $$0(orcid)0000-0002-7955-7164$$ade la Torre, Miriam$$uUniversidad de Zaragoza
000119999 700__ $$0(orcid)0000-0001-5193-7782$$aCalvo, Ana Cristina$$uUniversidad de Zaragoza
000119999 700__ $$0(orcid)0000-0003-0154-0730$$aPardo, Julián$$uUniversidad de Zaragoza
000119999 700__ $$0(orcid)0000-0001-5687-6704$$aOsta, Rosario$$uUniversidad de Zaragoza
000119999 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000119999 7102_ $$11011$$2566$$aUniversidad de Zaragoza$$bDpto. Microb.Ped.Radio.Sal.Pú.$$cÁrea Inmunología
000119999 7102_ $$11012$$2315$$aUniversidad de Zaragoza$$bDpto. Farmac.Fisiol.y Med.L.F.$$cÁrea Farmacología
000119999 773__ $$g23, 21 (2022), 13554 [15 pp.]$$pInt. j. mol. sci.$$tInternational Journal of Molecular Sciences$$x1661-6596
000119999 8564_ $$s3107747$$uhttps://zaguan.unizar.es/record/119999/files/texto_completo.pdf$$yVersión publicada
000119999 8564_ $$s2688978$$uhttps://zaguan.unizar.es/record/119999/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000119999 909CO $$ooai:zaguan.unizar.es:119999$$particulos$$pdriver
000119999 951__ $$a2024-03-18-12:34:12
000119999 980__ $$aARTICLE