120030 20240319081004.0 doi 10.1080/21505594.2022.2119657 sideral 130854 ART-2022-130854 eng Asai, Masanori Galleria mellonella as an infection model for the virulent Mycobacterium tuberculosis H37Rv 2022 Access copy available to the general public Unrestricted Tuberculosis (TB), caused by Mycobacterium tuberculosis (MTB), is a leading cause of infectious disease mortality. Animal infection models have contributed substantially to our understanding of TB, yet their biological and non-biological limitations are a research bottleneck. There is a need for more ethically acceptable, economical, and reproducible TB infection models capable of mimicking key aspects of disease. Here, we demonstrate and present a basic description of how Galleria mellonella (the greater wax moth, Gm) larvae can be used as a low cost, rapid, and ethically more acceptable model for TB research. This is the first study to infect Gm with the fully virulent MTB H37Rv, the most widely used strain in research. Infection of Gm with MTB resulted in a symptomatic lethal infection, the virulence of which differed from both attenuated Mycobacterium bovis BCG and auxotrophic MTB strains. The Gm-MTB model can also be used for anti-TB drug screening, although CFU enumeration from Gm is necessary for confirmation of mycobacterial load reducing activity of the tested compound. Furthermore, comparative virulence of MTB isogenic mutants can be determined in Gm. However, comparison of mutant phenotypes in Gm against conventional models must consider the limitations of innate immunity. Our findings indicate that Gm will be a practical, valuable, and advantageous additional model to be used alongside existing models to advance tuberculosis research. info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 5.2 2022 IMMUNOLOGY 64 / 161 = 0.398 2022 Q2 T2 MICROBIOLOGY 38 / 135 = 0.281 2022 Q2 T1 INFECTIOUS DISEASES 29 / 96 = 0.302 2022 Q2 T1 1.104 2022 Microbiology 2022 Q1 Infectious Diseases 2022 Q1 Parasitology 2022 Q1 Microbiology (medical) 2022 Q1 Immunology 2022 Q2 7.3 2022 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Li, Yanwen Spiropoulos, John Cooley, William Everest, David J. Kendall, Sharon L. Martín, Carlos Universidad de Zaragoza (orcid)0000-0003-2993-5478 Robertson, Brian D. Langford, Paul R. Newton, Sandra M. 1011 630 Universidad de Zaragoza Dpto. Microb.Ped.Radio.Sal.Pú. Área Microbiología 13, 1 (2022), 1543-1557 VIRULENCE Virulence 2150-5594 9569266 http://zaguan.unizar.es/record/120030/files/texto_completo.pdf Versión publicada 1145952 http://zaguan.unizar.es/record/120030/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:120030 articulos driver 2024-03-18-14:26:49 ARTICLE