120104 20241125101127.0 doi 10.1002/jbm.a.37448 sideral 130378 ART-2023-130378 eng Alamán-Díez, Pilar Universidad de Zaragoza (orcid)0000-0003-1958-4432 A bone-on-a-chip collagen hydrogel-based model using pre-differentiated adipose-derived stem cells for personalized bone tissue engineering 2023 Access copy available to the general public Unrestricted Mesenchymal stem cells have contributed to the continuous progress of tissue engineering and regenerative medicine. Adipose-derived stem cells (ADSC) possess many advantages compared to other origins including easy tissue harvesting, self-renewal potential, and fast population doubling time. As multipotent cells, they can differentiate into osteoblastic cell linages. In vitro bone models are needed to carry out an initial safety assessment in the study of novel bone regeneration therapies. We hypothesized that 3D bone-on-a-chip models containing ADSC could closely recreate the physiological bone microenvironment and promote differentiation. They represent an intermedium step between traditional 2D–in vitro and in vivo experiments facilitating the screening of therapeutic molecules while saving resources. Herein, we have differentiated ADSC for 7 and 14 days and used them to fabricate in vitro bone models by embedding the pre-differentiated cells in a 3D collagen matrix placed in a microfluidic chip. Osteogenic markers such as alkaline phosphatase activity, calcium mineralization, changes on cell morphology, and expression of specific proteins (bone sialoprotein 2, dentin matrix acidic phosphoprotein-1, and osteocalcin) were evaluated to determine cell differentiation potential and evolution. This is the first miniaturized 3D-in vitro bone model created from pre-differentiated ADSC embedded in a hydrogel collagen matrix which could be used for personalized bone tissue engineering. info:eu-repo/grantAgreement/ES/AEI/PID2020-113819RB-I00 info:eu-repo/grantAgreement/ES/MICINN/DPI2017-84780-C2-1-R info:eu-repo/semantics/openAccess by-nc http://creativecommons.org/licenses/by-nc/3.0/es/ 3.9 2023 ENGINEERING, BIOMEDICAL 42 / 123 = 0.341 2023 Q2 T2 MATERIALS SCIENCE, BIOMATERIALS 27 / 53 = 0.509 2023 Q3 T2 0.807 2023 Metals and Alloys 2023 Q1 Ceramics and Composites 2023 Q2 Biomaterials 2023 Q2 Biomedical Engineering 2023 Q2 10.4 2023 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion García-Gareta, Elena Universidad de Zaragoza (orcid)0000-0001-7062-9099 Arruebo, Manuel Universidad de Zaragoza (orcid)0000-0003-3165-0156 Pérez, María Ángeles Universidad de Zaragoza (orcid)0000-0002-2901-4188 5005 555 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Ingeniería Química 5004 605 Universidad de Zaragoza Dpto. Ingeniería Mecánica Área Mec.Med.Cont. y Teor.Est. 5001 065 Universidad de Zaragoza Dpto. Ciencia Tecnol.Mater.Fl. Área Cienc.Mater. Ingen.Metal. 111, 1 (2023), 88-105 J. Biomed. Mater. Res. Part A JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A 1549-3296 9036787 http://zaguan.unizar.es/record/120104/files/texto_completo.pdf Versión publicada 2333622 http://zaguan.unizar.es/record/120104/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:120104 articulos driver 2024-11-22-11:58:03 ARTICLE