000120171 001__ 120171
000120171 005__ 20240319081013.0
000120171 0247_ $$2doi$$a10.3390/ijms232214422
000120171 0248_ $$2sideral$$a131076
000120171 037__ $$aART-2022-131076
000120171 041__ $$aeng
000120171 100__ $$0(orcid)0000-0002-2231-7565$$aValero, Marta Sofía$$uUniversidad de Zaragoza
000120171 245__ $$aInvolvement of NO/cGMP Signaling Pathway, Ca2+ and K+ Channels on Spasmolytic Effect of Everlasting Flower Polyphenolic Extract (Helichrysum stoechas (L.) Moench)
000120171 260__ $$c2022
000120171 5060_ $$aAccess copy available to the general public$$fUnrestricted
000120171 5203_ $$aFunctional gastrointestinal diseases (FGID) are worldwide prevalent conditions. Pharmacological treatments can be ineffective, leading the population to turn to herbal or traditional remedies. Helichrysum stoechas (L.) Moench is a medicinal plant traditionally used in the Iberian Peninsula to treat digestive disorders, but its effects on gastrointestinal motility have not been scientifically demonstrated. The aim of this work was to evaluate the antispasmodic effect of a polyphenolic extract of H. stoechas (HSM), its mechanism of action and its antioxidant activity. Isometric myography studies were performed in rat ileum, and malondialdehyde (MDA) and 4-hydroxyalkenals (4-HDA) levels were measured in rat jejunum. HSM reduced the integrated mechanical activity of spontaneous contractions. In Ca2+-free medium, HSM reduced the concentration–response curve of CaCl2 similarly to verapamil. Pre-incubation with the extract blocked the contraction induced by Bay K8644, KCl and carbachol. L-NAME, ODQ, Rp-8-Br-PET-cGMPS, KT-5823, apamin, TRAM-34 and charybdotoxin reduced the relaxant effect of the extract on spontaneous contractions. MDA+4-HDA levels in LPS-treated tissue were reduced by the extract, showing antioxidant activity. In conclusion, HSM showed antispasmodic activity through inhibition of Ca2+ influx, activation of the NO/PKG/cGMP pathway and opening of Ca2+-activated K+ channels. The results suggest that H. stoechas could help in the prevention or treatment of FGIDs.
000120171 536__ $$9info:eu-repo/grantAgreement/ES/DGA/B44-20D$$9info:eu-repo/grantAgreement/ES/UZ/JIUZ-2018-BIO-09$$9info:eu-repo/grantAgreement/ES/UZ/JIUZ-2021-BIO-08
000120171 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000120171 590__ $$a5.6$$b2022
000120171 592__ $$a1.154$$b2022
000120171 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b66 / 285 = 0.232$$c2022$$dQ1$$eT1
000120171 593__ $$aMedicine (miscellaneous)$$c2022$$dQ1
000120171 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b52 / 178 = 0.292$$c2022$$dQ2$$eT1
000120171 593__ $$aPhysical and Theoretical Chemistry$$c2022$$dQ1
000120171 593__ $$aComputer Science Applications$$c2022$$dQ1
000120171 593__ $$aInorganic Chemistry$$c2022$$dQ1
000120171 593__ $$aSpectroscopy$$c2022$$dQ1
000120171 593__ $$aOrganic Chemistry$$c2022$$dQ1
000120171 593__ $$aMolecular Biology$$c2022$$dQ2
000120171 593__ $$aCatalysis$$c2022$$dQ2
000120171 594__ $$a7.8$$b2022
000120171 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000120171 700__ $$0(orcid)0000-0001-6969-1055$$aLópez, Víctor
000120171 700__ $$0(orcid)0000-0001-8584-3979$$aCastro, Marta$$uUniversidad de Zaragoza
000120171 700__ $$aGómez-Rincón, Carlota
000120171 700__ $$0(orcid)0000-0002-2114-0577$$aArruebo, María Pilar$$uUniversidad de Zaragoza
000120171 700__ $$0(orcid)0000-0002-7972-7119$$aLes, Francisco
000120171 700__ $$0(orcid)0000-0002-7412-2073$$aPlaza, Miguel Ángel$$uUniversidad de Zaragoza
000120171 7102_ $$11012$$2410$$aUniversidad de Zaragoza$$bDpto. Farmac.Fisiol.y Med.L.F.$$cÁrea Fisiología
000120171 773__ $$g23, 22 (2022), 14422 [17 pp.]$$pInt. j. mol. sci.$$tInternational Journal of Molecular Sciences$$x1661-6596
000120171 8564_ $$s3062710$$uhttps://zaguan.unizar.es/record/120171/files/texto_completo.pdf$$yVersión publicada
000120171 8564_ $$s2865930$$uhttps://zaguan.unizar.es/record/120171/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000120171 909CO $$ooai:zaguan.unizar.es:120171$$particulos$$pdriver
000120171 951__ $$a2024-03-18-15:20:57
000120171 980__ $$aARTICLE