120171 20240319081013.0 doi 10.3390/ijms232214422 sideral 131076 ART-2022-131076 eng Valero, Marta Sofía Universidad de Zaragoza (orcid)0000-0002-2231-7565 Involvement of NO/cGMP Signaling Pathway, Ca2+ and K+ Channels on Spasmolytic Effect of Everlasting Flower Polyphenolic Extract (Helichrysum stoechas (L.) Moench) 2022 Access copy available to the general public Unrestricted Functional gastrointestinal diseases (FGID) are worldwide prevalent conditions. Pharmacological treatments can be ineffective, leading the population to turn to herbal or traditional remedies. Helichrysum stoechas (L.) Moench is a medicinal plant traditionally used in the Iberian Peninsula to treat digestive disorders, but its effects on gastrointestinal motility have not been scientifically demonstrated. The aim of this work was to evaluate the antispasmodic effect of a polyphenolic extract of H. stoechas (HSM), its mechanism of action and its antioxidant activity. Isometric myography studies were performed in rat ileum, and malondialdehyde (MDA) and 4-hydroxyalkenals (4-HDA) levels were measured in rat jejunum. HSM reduced the integrated mechanical activity of spontaneous contractions. In Ca2+-free medium, HSM reduced the concentration–response curve of CaCl2 similarly to verapamil. Pre-incubation with the extract blocked the contraction induced by Bay K8644, KCl and carbachol. L-NAME, ODQ, Rp-8-Br-PET-cGMPS, KT-5823, apamin, TRAM-34 and charybdotoxin reduced the relaxant effect of the extract on spontaneous contractions. MDA+4-HDA levels in LPS-treated tissue were reduced by the extract, showing antioxidant activity. In conclusion, HSM showed antispasmodic activity through inhibition of Ca2+ influx, activation of the NO/PKG/cGMP pathway and opening of Ca2+-activated K+ channels. The results suggest that H. stoechas could help in the prevention or treatment of FGIDs. info:eu-repo/grantAgreement/ES/DGA/B44-20D info:eu-repo/grantAgreement/ES/UZ/JIUZ-2018-BIO-09 info:eu-repo/grantAgreement/ES/UZ/JIUZ-2021-BIO-08 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 5.6 2022 BIOCHEMISTRY & MOLECULAR BIOLOGY 66 / 285 = 0.232 2022 Q1 T1 CHEMISTRY, MULTIDISCIPLINARY 52 / 178 = 0.292 2022 Q2 T1 1.154 2022 Medicine (miscellaneous) 2022 Q1 Physical and Theoretical Chemistry 2022 Q1 Computer Science Applications 2022 Q1 Inorganic Chemistry 2022 Q1 Spectroscopy 2022 Q1 Organic Chemistry 2022 Q1 Molecular Biology 2022 Q2 Catalysis 2022 Q2 7.8 2022 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion López, Víctor (orcid)0000-0001-6969-1055 Castro, Marta Universidad de Zaragoza (orcid)0000-0001-8584-3979 Gómez-Rincón, Carlota Arruebo, María Pilar Universidad de Zaragoza (orcid)0000-0002-2114-0577 Les, Francisco (orcid)0000-0002-7972-7119 Plaza, Miguel Ángel Universidad de Zaragoza (orcid)0000-0002-7412-2073 1012 410 Universidad de Zaragoza Dpto. Farmac.Fisiol.y Med.L.F. Área Fisiología 23, 22 (2022), 14422 [17 pp.] Int. j. mol. sci. International Journal of Molecular Sciences 1661-6596 3062710 http://zaguan.unizar.es/record/120171/files/texto_completo.pdf Versión publicada 2865930 http://zaguan.unizar.es/record/120171/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:120171 articulos driver 2024-03-18-15:20:57 ARTICLE