000120172 001__ 120172
000120172 005__ 20240319081013.0
000120172 0247_ $$2doi$$a10.3390/plants11162101
000120172 0248_ $$2sideral$$a131208
000120172 037__ $$aART-2022-131208
000120172 041__ $$aeng
000120172 100__ $$0(orcid)0000-0001-6435-3540$$aSevilla, Emma$$uUniversidad de Zaragoza
000120172 245__ $$aIdentification of Early Salt-Stress-Responsive Proteins in In Vitro Prunus Cultured Excised Roots
000120172 260__ $$c2022
000120172 5060_ $$aAccess copy available to the general public$$fUnrestricted
000120172 5203_ $$aFruit-tree rootstock selection is a challenge under a scenario of growing environmental stresses in which the soil and climate are greatly affected. Salinization is an increasing global process that severely affects soil fertility. The selection of rootstocks with the ability to tolerate salt stress is essential. Excised root cultures may be an excellent experimental approach to study stress physiology and a predictive tool to assess possible tolerance. In this study, we show how protein changes in response to salt stress evaluated in excised root cultures of Prunus cerasus (moderate salt-sensitive cultivar) could be representative of these changes in the roots of whole plants. The 2D electrophoresis of root extracts and subsequent spot identification by MALDI-TOF/TOF-MS show 16 relevant proteins differentially expressed in roots as a response to 60 mM NaCl. Cytoplasmic isozyme fructose 1,6-bisphosphate aldolase shows relevant changes in its relative presence of isoforms as a response to saline stress, while the total level of enzymes remains similar. Ferredoxin-NADP+ reductase increases as a response to salinity, even though the measured activity is not significantly different. The observed changes are congruent with previous proteomic studies on the roots of whole plants that are involved in protection mechanisms against salt stress.
000120172 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A12-17R$$9info:eu-repo/grantAgreement/ES/DGA/E35-17R$$9info:eu-repo/grantAgreement/ES/MCIU/PID2019-104889GB-I00$$9info:eu-repo/grantAgreement/ES/MINECO/BFU2016-77671-P$$9info:eu-repo/grantAgreement/ES/MINECO/RTA-PID2019-103985RR-I00
000120172 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000120172 590__ $$a4.5$$b2022
000120172 592__ $$a0.79$$b2022
000120172 591__ $$aPLANT SCIENCES$$b43 / 239 = 0.18$$c2022$$dQ1$$eT1
000120172 593__ $$aEcology$$c2022$$dQ1
000120172 593__ $$aPlant Science$$c2022$$dQ1
000120172 593__ $$aEcology, Evolution, Behavior and Systematics$$c2022$$dQ1
000120172 594__ $$a5.4$$b2022
000120172 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000120172 700__ $$aAndreu, Pilar
000120172 700__ $$0(orcid)0000-0001-8644-4574$$aFillat, María F.$$uUniversidad de Zaragoza
000120172 700__ $$0(orcid)0000-0002-2742-3711$$aPeleato, M. Luisa$$uUniversidad de Zaragoza
000120172 700__ $$aMarín, Juan A.
000120172 700__ $$aArbeloa, Arancha
000120172 7102_ $$11002$$2412$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Fisiología Vegetal
000120172 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000120172 773__ $$g11, 16 (2022), 2101 [13 pp.]$$tPlants$$x2223-7747
000120172 8564_ $$s2800763$$uhttps://zaguan.unizar.es/record/120172/files/texto_completo.pdf$$yVersión publicada
000120172 8564_ $$s2775940$$uhttps://zaguan.unizar.es/record/120172/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000120172 909CO $$ooai:zaguan.unizar.es:120172$$particulos$$pdriver
000120172 951__ $$a2024-03-18-15:21:05
000120172 980__ $$aARTICLE