000120219 001__ 120219
000120219 005__ 20240319081022.0
000120219 0247_ $$2doi$$a10.1016/j.mimet.2022.106618
000120219 0248_ $$2sideral$$a131212
000120219 037__ $$aART-2022-131212
000120219 041__ $$aeng
000120219 100__ $$0(orcid)0000-0002-9181-2105$$aGimenez-Ingalaturre, Ana C.$$uUniversidad de Zaragoza
000120219 245__ $$aContribution to optimization and standardization of antibacterial assays with silver nanoparticles: the culture medium and their aggregation
000120219 260__ $$c2022
000120219 5060_ $$aAccess copy available to the general public$$fUnrestricted
000120219 5203_ $$aThe antimicrobial activity of silver nanoparticles is determined by their size and specific properties, as well as by the chemical composition of the exposure medium in which the nanoparticles are suspended. When the antibacterial tests are carried out in a culture medium, aggregation of the nanoparticles is produced, decreasing their effectiveness. This study proposes the addition of surfactants to the culture medium to prevent the aggregation of silver nanoparticles and optimizes the concentrations of these surfactants. The aggregation of silver nanoparticles was studied by dynamic light scattering (DLS) after dispersion in three liquid culture media (Mueller-Hinton (MH), Luria-Bertani (LB) and Brain Heart Infusion) in which four different surfactants (SDS, Triton X100, Tween 80 and CTAB) were added at concentrations of 0, 0.1, 0.5, 1, 1.5 and 2%. Results showed that, the optimal culture media to prevent aggregation of silver nanoparticles were MH and LB with higher concentrations of Tween 80 and Triton X100 surfactants; being MH + 2% of Tween 80 and MH + 1% Triton X100 the best combinations obtained because the results obtained were closest to the sizes of nanoparticles in ultrapure water. In addition, it has been verified that the optimal medium + surfactant combinations chosen did not affect the viability of Escherichia coli bacteria. Nanoparticle aggregation was not observed by single particle inductively coupled plasma mass spectrometry (SP-ICP-MS) when nanoparticles were incubated for long incubations periods (24 h) in the optimal medium chosen.
000120219 536__ $$9info:eu-repo/grantAgreement/ES/DGA/B43-20R Grupo Agua y salud$$9info:eu-repo/grantAgreement/ES/DGA/E29-20R$$9info:eu-repo/grantAgreement/ES/MCIU-FEDER/RTI2018-096111-B-I00
000120219 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000120219 590__ $$a2.2$$b2022
000120219 592__ $$a0.467$$b2022
000120219 591__ $$aBIOCHEMICAL RESEARCH METHODS$$b55 / 77 = 0.714$$c2022$$dQ3$$eT3
000120219 593__ $$aMicrobiology$$c2022$$dQ3
000120219 591__ $$aMICROBIOLOGY$$b114 / 135 = 0.844$$c2022$$dQ4$$eT3
000120219 593__ $$aMicrobiology (medical)$$c2022$$dQ3
000120219 593__ $$aMolecular Biology$$c2022$$dQ4
000120219 594__ $$a5.0$$b2022
000120219 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000120219 700__ $$0(orcid)0000-0002-9273-5885$$aRubio, Encarnación$$uUniversidad de Zaragoza
000120219 700__ $$0(orcid)0000-0002-9981-9045$$aChueca, Patricia$$uUniversidad de Zaragoza
000120219 700__ $$0(orcid)0000-0002-4169-0357$$aLaborda, Francisco$$uUniversidad de Zaragoza
000120219 700__ $$0(orcid)0000-0003-0765-7227$$aGoñi, Pilar$$uUniversidad de Zaragoza
000120219 7102_ $$11011$$2660$$aUniversidad de Zaragoza$$bDpto. Microb.Ped.Radio.Sal.Pú.$$cÁrea Parasitología
000120219 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica
000120219 7102_ $$11011$$2X$$aUniversidad de Zaragoza$$bDpto. Microb.Ped.Radio.Sal.Pú.$$cÁrea Técnica. Lab. y Talleres
000120219 7102_ $$11011$$2615$$aUniversidad de Zaragoza$$bDpto. Microb.Ped.Radio.Sal.Pú.$$cÁrea Medic.Prevent.Salud Públ.
000120219 773__ $$g203 (2022), 106618 [7 pp.]$$pJ. microbiol. methods$$tJOURNAL OF MICROBIOLOGICAL METHODS$$x0167-7012
000120219 8564_ $$s2144187$$uhttps://zaguan.unizar.es/record/120219/files/texto_completo.pdf$$yVersión publicada
000120219 8564_ $$s2580971$$uhttps://zaguan.unizar.es/record/120219/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000120219 909CO $$ooai:zaguan.unizar.es:120219$$particulos$$pdriver
000120219 951__ $$a2024-03-18-16:20:58
000120219 980__ $$aARTICLE