120219 20240319081022.0 doi 10.1016/j.mimet.2022.106618 sideral 131212 ART-2022-131212 eng Gimenez-Ingalaturre, Ana C. Universidad de Zaragoza (orcid)0000-0002-9181-2105 Contribution to optimization and standardization of antibacterial assays with silver nanoparticles: the culture medium and their aggregation 2022 Access copy available to the general public Unrestricted The antimicrobial activity of silver nanoparticles is determined by their size and specific properties, as well as by the chemical composition of the exposure medium in which the nanoparticles are suspended. When the antibacterial tests are carried out in a culture medium, aggregation of the nanoparticles is produced, decreasing their effectiveness. This study proposes the addition of surfactants to the culture medium to prevent the aggregation of silver nanoparticles and optimizes the concentrations of these surfactants. The aggregation of silver nanoparticles was studied by dynamic light scattering (DLS) after dispersion in three liquid culture media (Mueller-Hinton (MH), Luria-Bertani (LB) and Brain Heart Infusion) in which four different surfactants (SDS, Triton X100, Tween 80 and CTAB) were added at concentrations of 0, 0.1, 0.5, 1, 1.5 and 2%. Results showed that, the optimal culture media to prevent aggregation of silver nanoparticles were MH and LB with higher concentrations of Tween 80 and Triton X100 surfactants; being MH + 2% of Tween 80 and MH + 1% Triton X100 the best combinations obtained because the results obtained were closest to the sizes of nanoparticles in ultrapure water. In addition, it has been verified that the optimal medium + surfactant combinations chosen did not affect the viability of Escherichia coli bacteria. Nanoparticle aggregation was not observed by single particle inductively coupled plasma mass spectrometry (SP-ICP-MS) when nanoparticles were incubated for long incubations periods (24 h) in the optimal medium chosen. info:eu-repo/grantAgreement/ES/DGA/B43-20R Grupo Agua y salud info:eu-repo/grantAgreement/ES/DGA/E29-20R info:eu-repo/grantAgreement/ES/MCIU-FEDER/RTI2018-096111-B-I00 info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 2.2 2022 BIOCHEMICAL RESEARCH METHODS 55 / 77 = 0.714 2022 Q3 T3 MICROBIOLOGY 114 / 135 = 0.844 2022 Q4 T3 0.467 2022 Microbiology 2022 Q3 Microbiology (medical) 2022 Q3 Molecular Biology 2022 Q4 5.0 2022 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Rubio, Encarnación Universidad de Zaragoza (orcid)0000-0002-9273-5885 Chueca, Patricia Universidad de Zaragoza (orcid)0000-0002-9981-9045 Laborda, Francisco Universidad de Zaragoza (orcid)0000-0002-4169-0357 Goñi, Pilar Universidad de Zaragoza (orcid)0000-0003-0765-7227 1011 660 Universidad de Zaragoza Dpto. Microb.Ped.Radio.Sal.Pú. Área Parasitología 2009 750 Universidad de Zaragoza Dpto. Química Analítica Área Química Analítica 1011 X Universidad de Zaragoza Dpto. Microb.Ped.Radio.Sal.Pú. Área Técnica. Lab. y Talleres 1011 615 Universidad de Zaragoza Dpto. Microb.Ped.Radio.Sal.Pú. Área Medic.Prevent.Salud Públ. 203 (2022), 106618 [7 pp.] J. microbiol. methods JOURNAL OF MICROBIOLOGICAL METHODS 0167-7012 2144187 http://zaguan.unizar.es/record/120219/files/texto_completo.pdf Versión publicada 2580971 http://zaguan.unizar.es/record/120219/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:120219 articulos driver 2024-03-18-16:20:58 ARTICLE