000120249 001__ 120249
000120249 005__ 20240319081026.0
000120249 0247_ $$2doi$$a10.3389/fvets.2022.1014648
000120249 0248_ $$2sideral$$a131217
000120249 037__ $$aART-2022-131217
000120249 041__ $$aeng
000120249 100__ $$0(orcid)0000-0002-7453-1766$$aMonleón, E.$$uUniversidad de Zaragoza
000120249 245__ $$aLiver tissue remodeling following ablation with irreversible electroporation in a porcine model
000120249 260__ $$c2022
000120249 5060_ $$aAccess copy available to the general public$$fUnrestricted
000120249 5203_ $$aIrreversible electroporation (IRE) is a method of non-thermal focal tissue ablation characterized by irreversibly permeabilizing the cell membranes while preserving the extracellular matrix. This study aimed to investigate tissue remodeling after IRE in a porcine model, especially focusing on the extracellular matrix and hepatic stellate cells. IRE ablation was performed on 11 female pigs at 2,000 V/cm electric field strength using a versatile high-voltage generator and 3 cm diameter parallel-plate electrodes. The treated lobes were removed during surgery at 1, 3, 7, 14, and 21 days after IRE. Tissue remodeling and regeneration were assessed by histopathology and immunohistochemistry. Throughout the treated area, IRE led to extensive necrosis with intact collagenous structures evident until day 1. From then on, the necrosis progressively diminished while reparative tissue gradually increased. During this process, the reticulin framework and the septal fibrillar collagen remained in the necrotic foci until they were invaded by the reparative tissue. The reparative tissue was characterized by a massive proliferation of myofibroblast-like cells accompanied by a complete disorganization of the extracellular matrix with the disappearance of hepatic architecture. Hepatic stellate cell markers were associated with the proliferation of myofibroblast-like cells and the reorganization of the extracellular matrix. Between 2 and 3 weeks after IRE, the lobular architecture was almost completely regenerated. The events described in the present study show that IRE may be a valid model to study the mechanisms underlying liver regeneration after extensive acute injury.
000120249 536__ $$9info:eu-repo/grantAgreement/ES/DGA/LMP106-18$$9info:eu-repo/grantAgreement/ES/ISCIII/PI21-00440$$9info:eu-repo/grantAgreement/ES/MICINN/PID2019-103939RB-I00
000120249 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000120249 590__ $$a3.2$$b2022
000120249 592__ $$a0.737$$b2022
000120249 591__ $$aVETERINARY SCIENCES$$b12 / 144 = 0.083$$c2022$$dQ1$$eT1
000120249 593__ $$aVeterinary (miscellaneous)$$c2022$$dQ1
000120249 594__ $$a3.8$$b2022
000120249 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000120249 700__ $$0(orcid)0000-0002-1284-9007$$aLucía, Ó.$$uUniversidad de Zaragoza
000120249 700__ $$0(orcid)0000-0002-4437-2581$$aGüemes, A.$$uUniversidad de Zaragoza
000120249 700__ $$0(orcid)0000-0003-2848-170X$$aLópez-Alonso, B.$$uUniversidad de Zaragoza
000120249 700__ $$0(orcid)0000-0002-3592-820X$$aArribas, D.$$uUniversidad de Zaragoza
000120249 700__ $$0(orcid)0000-0001-8399-4650$$aSarnago, H.$$uUniversidad de Zaragoza
000120249 700__ $$aHernáez, A.
000120249 700__ $$0(orcid)0000-0002-9655-5531$$aBurdío, J. M.$$uUniversidad de Zaragoza
000120249 700__ $$0(orcid)0000-0002-9951-1075$$aJunquera, C.$$uUniversidad de Zaragoza
000120249 7102_ $$11003$$2443$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Histología
000120249 7102_ $$15008$$2785$$aUniversidad de Zaragoza$$bDpto. Ingeniería Electrón.Com.$$cÁrea Tecnología Electrónica
000120249 7102_ $$11013$$2090$$aUniversidad de Zaragoza$$bDpto. Cirugía$$cÁrea Cirugía
000120249 773__ $$g9 (2022), 1014648[11 pp.]$$pFront. vet. sci.$$tFrontiers in Veterinary Science$$x2297-1769
000120249 8564_ $$s6128965$$uhttps://zaguan.unizar.es/record/120249/files/texto_completo.pdf$$yVersión publicada
000120249 8564_ $$s2136224$$uhttps://zaguan.unizar.es/record/120249/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000120249 909CO $$ooai:zaguan.unizar.es:120249$$particulos$$pdriver
000120249 951__ $$a2024-03-18-16:44:04
000120249 980__ $$aARTICLE