000120894 001__ 120894
000120894 005__ 20240202150202.0
000120894 0247_ $$2doi$$a10.1016/j.chroma.2021.461895
000120894 0248_ $$2sideral$$a123183
000120894 037__ $$aART-2021-123183
000120894 041__ $$aeng
000120894 100__ $$0(orcid)0000-0002-8701-9745$$aJarne, C.
000120894 245__ $$aGlobotriaosylceramide-related biomarkers of fabry disease identified in plasma by high-performance thin-layer chromatography - densitometry- mass spectrometry
000120894 260__ $$c2021
000120894 5060_ $$aAccess copy available to the general public$$fUnrestricted
000120894 5203_ $$aIdentification of 19 molecular species of globotriaosylceramides (Gb3) in extracts from a Fabry''s plasma patient and a healthy control was performed by High-Performance Thin-Layer Chromatography (HPTLC)-densitometry and online coupling to Mass Spectrometry (MS). Separation was carried out on LiChrospher plates using Automated Multiple Development (AMD). Densitometry was performed on twin plates by combining detection in the visible at 550 nm, through previous on-plate orcinol derivatization, and by Ultraviolet 190 nm, using a non-impregnated plate. The latter was directly coupled to an ion-trap mass spectrometer through an automated elution-based interface. Gb3 molecular species, which were identified by HPTLC- Electrospray Mass Spectrometry (+)-MS and confirmed by MS/MS or HPTLC-Atmospheric Pressure Chemical Ionization Mass Spectrometry (+)-MS, are: five isoforms of saturated Gb3; seven isoforms of methylated Gb3; and seven species with two additional double bonds. Twelve of these species were previously reported as biomarkers of Fabry''s lysosomal disorder using a Liquid Chromatography-MS-based method, and the other seven are structurally similar, closely related to them. Saturated Gb3 isoforms migrated on LiChrospher plate in one of the separated peaks corresponding to the migration zone of ceramide trihexosides standard. Instead, methylated and unsaturated Gb3 species co-migrated with sphingomyelin species. Ion intensity ESI-MS profiles show that saturated Gb3 species in Fabry''s plasma were in higher concentration than in control sample. Before applying the Thin-Layer Chromatography (TLC)-MS interface on HPTLC separated peaks, its positioning precision was first studied using ceramide tri-hexosides as model compound. This provided information on Gb3 peak broadening and splitting during its migration.
000120894 536__ $$9info:eu-repo/grantAgreement/ES/DGA/E25-17R$$9info:eu-repo/grantAgreement/ES/MINECO/CTQ2016-76846-R
000120894 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000120894 590__ $$a4.601$$b2021
000120894 591__ $$aCHEMISTRY, ANALYTICAL$$b20 / 87 = 0.23$$c2021$$dQ1$$eT1
000120894 591__ $$aBIOCHEMICAL RESEARCH METHODS$$b23 / 79 = 0.291$$c2021$$dQ2$$eT1
000120894 592__ $$a0.813$$b2021
000120894 593__ $$aAnalytical Chemistry$$c2021$$dQ1
000120894 593__ $$aOrganic Chemistry$$c2021$$dQ1
000120894 593__ $$aMedicine (miscellaneous)$$c2021$$dQ1
000120894 594__ $$a7.3$$b2021
000120894 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000120894 700__ $$aMembrado, L.
000120894 700__ $$0(orcid)0000-0001-7299-0187$$aSavirón, M.
000120894 700__ $$0(orcid)0000-0002-4887-1652$$aVela, J.$$uUniversidad de Zaragoza
000120894 700__ $$0(orcid)0000-0003-3514-2570$$aOrduna, J.
000120894 700__ $$0(orcid)0000-0003-2607-7834$$aGarriga, R.$$uUniversidad de Zaragoza
000120894 700__ $$0(orcid)0000-0002-8973-5104$$aGalbán, J.$$uUniversidad de Zaragoza
000120894 700__ $$aCebolla, V.L.
000120894 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica
000120894 7102_ $$12012$$2755$$aUniversidad de Zaragoza$$bDpto. Química Física$$cÁrea Química Física
000120894 773__ $$g1638 (2021), 461895 [11 pp.]$$pJ. chromatogr. A$$tJournal of Chromatography A$$x0021-9673
000120894 8564_ $$s860805$$uhttps://zaguan.unizar.es/record/120894/files/texto_completo.pdf$$yPostprint
000120894 8564_ $$s2066275$$uhttps://zaguan.unizar.es/record/120894/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000120894 909CO $$ooai:zaguan.unizar.es:120894$$particulos$$pdriver
000120894 951__ $$a2024-02-02-14:58:14
000120894 980__ $$aARTICLE