120894 20240202150202.0 doi 10.1016/j.chroma.2021.461895 sideral 123183 ART-2021-123183 eng Jarne, C. (orcid)0000-0002-8701-9745 Globotriaosylceramide-related biomarkers of fabry disease identified in plasma by high-performance thin-layer chromatography - densitometry- mass spectrometry 2021 Access copy available to the general public Unrestricted Identification of 19 molecular species of globotriaosylceramides (Gb3) in extracts from a Fabry''s plasma patient and a healthy control was performed by High-Performance Thin-Layer Chromatography (HPTLC)-densitometry and online coupling to Mass Spectrometry (MS). Separation was carried out on LiChrospher plates using Automated Multiple Development (AMD). Densitometry was performed on twin plates by combining detection in the visible at 550 nm, through previous on-plate orcinol derivatization, and by Ultraviolet 190 nm, using a non-impregnated plate. The latter was directly coupled to an ion-trap mass spectrometer through an automated elution-based interface. Gb3 molecular species, which were identified by HPTLC- Electrospray Mass Spectrometry (+)-MS and confirmed by MS/MS or HPTLC-Atmospheric Pressure Chemical Ionization Mass Spectrometry (+)-MS, are: five isoforms of saturated Gb3; seven isoforms of methylated Gb3; and seven species with two additional double bonds. Twelve of these species were previously reported as biomarkers of Fabry''s lysosomal disorder using a Liquid Chromatography-MS-based method, and the other seven are structurally similar, closely related to them. Saturated Gb3 isoforms migrated on LiChrospher plate in one of the separated peaks corresponding to the migration zone of ceramide trihexosides standard. Instead, methylated and unsaturated Gb3 species co-migrated with sphingomyelin species. Ion intensity ESI-MS profiles show that saturated Gb3 species in Fabry''s plasma were in higher concentration than in control sample. Before applying the Thin-Layer Chromatography (TLC)-MS interface on HPTLC separated peaks, its positioning precision was first studied using ceramide tri-hexosides as model compound. This provided information on Gb3 peak broadening and splitting during its migration. info:eu-repo/grantAgreement/ES/DGA/E25-17R info:eu-repo/grantAgreement/ES/MINECO/CTQ2016-76846-R info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 4.601 2021 CHEMISTRY, ANALYTICAL 20 / 87 = 0.23 2021 Q1 T1 BIOCHEMICAL RESEARCH METHODS 23 / 79 = 0.291 2021 Q2 T1 0.813 2021 Analytical Chemistry 2021 Q1 Organic Chemistry 2021 Q1 Medicine (miscellaneous) 2021 Q1 7.3 2021 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Membrado, L. Savirón, M. (orcid)0000-0001-7299-0187 Vela, J. Universidad de Zaragoza (orcid)0000-0002-4887-1652 Orduna, J. (orcid)0000-0003-3514-2570 Garriga, R. Universidad de Zaragoza (orcid)0000-0003-2607-7834 Galbán, J. Universidad de Zaragoza (orcid)0000-0002-8973-5104 Cebolla, V.L. 2009 750 Universidad de Zaragoza Dpto. Química Analítica Área Química Analítica 2012 755 Universidad de Zaragoza Dpto. Química Física Área Química Física 1638 (2021), 461895 [11 pp.] J. chromatogr. A Journal of Chromatography A 0021-9673 860805 http://zaguan.unizar.es/record/120894/files/texto_completo.pdf Postprint 2066275 http://zaguan.unizar.es/record/120894/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:120894 articulos driver 2024-02-02-14:58:14 ARTICLE