120965 20241125101126.0 doi 10.1177/03009858221137020 sideral 131340 ART-2023-131340 eng Moreno, B. Universidad de Zaragoza (orcid)0000-0001-6629-6842 Peripheral neuropathy caused by fenugreek (Trigonella foenum-graecum) straw intoxication in cattle and experimental reproduction in sheep and goats 2023 Access copy available to the general public Unrestricted Trigonella foenum-graecum (fenugreek) is a legume widely used as a food supplement in humans and less frequently in ruminants. Toxicity has been described sporadically in ruminants grazing mature fenugreek plants or stubble; however, the pathological features are unclear. This report describes a natural outbreak of intoxication in cattle fed fenugreek straw and the experimental reproduction using 8 sheep and 8 goats. Affected cattle presented clinical signs approximately 1 month after consuming the straw and 100 of 400 cattle (25%) were affected, of which 60 of 100 (60%) died or were euthanized. Clinical signs were characterized by proprioceptive positioning defects with abnormal postures and weakness of hindlimbs. Forelimbs were also affected in severely affected animals, and cattle became recumbent. Locomotion was characterized by trembling, and some cattle showed high-stepping movements of their forelimbs and knuckled over in their fetlocks. Experimental intoxication induced clinical signs only in sheep and were similar to cattle, although with signs starting in the forelegs. Gross and microscopic lesions were similar in spontaneous and experimental intoxications. Macroscopic changes corresponded with muscular hemorrhages and edema, mainly surrounding the peripheral nerves. Microscopic examination only demonstrated lesions in the distal peripheral nerves, which included edema, hemorrhages, and Wallerian degeneration. Neurofilament immunohistochemistry revealed altered axon labeling and S100 showed a decrease in myelin intensity and loss of its typical compact arrangement around axons. Biochemical and hematological abnormalities included elevated levels of muscle and liver enzymes and thrombocytopenia. These findings indicate that fenugreek straw induces peripheral neuropathy in cattle and sheep, but not in goats. info:eu-repo/grantAgreement/ES/DGA/A05-17R info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 2.3 2023 VETERINARY SCIENCES 28 / 167 = 0.168 2023 Q1 T1 PATHOLOGY 42 / 88 = 0.477 2023 Q2 T2 0.749 2023 Veterinary (miscellaneous) 2023 Q1 4.7 2023 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Marín, B. Universidad de Zaragoza (orcid)0000-0002-1590-3347 Otero, A. Universidad de Zaragoza (orcid)0000-0001-9075-2764 García, M. Universidad de Zaragoza (orcid)0000-0002-8752-5146 Raksa, H. Guijarro, M. I. Climent, M. Universidad de Zaragoza (orcid)0000-0002-0606-0763 Morales, M. Universidad de Zaragoza (orcid)0000-0001-9778-6545 Zabala, J. Loste, J. M. Acín, C. Universidad de Zaragoza (orcid)0000-0001-5105-6133 Badiola, J. J. Universidad de Zaragoza (orcid)0000-0002-7173-7216 1001 420 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Genética 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 1001 025 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Anatom.Anatom.Patológ.Com 60, 1 (2023), 115-122 Vet. pathol. Veterinary Pathology 0300-9858 4216039 http://zaguan.unizar.es/record/120965/files/texto_completo.pdf Postprint 1691515 http://zaguan.unizar.es/record/120965/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:120965 articulos driver 2024-11-22-11:57:44 ARTICLE