120995 20241125101127.0 doi 10.1007/s10534-022-00459-5 sideral 131308 ART-2023-131308 eng Abad, Inés Universidad de Zaragoza Effect of in vitro gastrointestinal digestion on the antibacterial activity of bioactive dairy formulas supplemented with lactoferrin against Cronobacter sakazakii 2023 Access copy available to the general public Unrestricted Milk is a source of proteins with high nutritional value and relevant biological activities. Bioactive milk proteins, like lactoferrin, are important for newborn development and can also be used as ingredients in functional products to improve health. Lactoferrin is essential in infant’s diet, since protects against infections and promotes immune system maturation. Bovine lactoferrin is used to supplement formula milk in order to strengthen baby’s defences against some pathogenic bacteria. Thus, lactoferrin supplemented formula can be a barrier against emergent pathogens, such as Cronobacter sakazakii, which has caused great concern in the last few years. Milk proteins generate bioactive peptides in the digestion process, and it is known that industrial processing can modify their susceptibility to digestion. Treatments such as heating have been shown to denature whey proteins and make them more easily digestible. Therefore, the aim of this study was to analyze the effect of technological treatments and gastrointestinal digestion on the antibacterial activity against C. sakazakii of proteins present in dairy formulas supplemented with lactoferrin. Commercial bovine lactoferrin has been shown to have antibacterial activity against C. sakazakii, both in the native state and after static in vitro gastrointestinal digestion. In addition, the digests obtained from dairy formulas subjected to technological treatments, either homogenization or pasteurization, have higher antibacterial activity than non-treated formulas. The release of low molecular weight peptides during the in vitro gastric digestion is probably the cause that would explain the enhanced antibacterial activity of the digested dairy formulas. info:eu-repo/grantAgreement/ES/DGA-FEDER/A02-20R info:eu-repo/grantAgreement/EC/H2020/801586/EU/International Doctoral Programme for Talent Attraction to the Campus of International Excellence of the Ebro Valley/IberusTalent This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 801586-IberusTalent info:eu-repo/grantAgreement/ES/MINECO/AGL2017-82987 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 4.1 2023 BIOCHEMISTRY & MOLECULAR BIOLOGY 97 / 313 = 0.31 2023 Q2 T1 0.618 2023 Agricultural and Biological Sciences (miscellaneous) 2023 Q1 Metals and Alloys 2023 Q1 Biochemistry, Genetics and Molecular Biology (miscellaneous) 2023 Q2 Biomaterials 2023 Q3 5.9 2023 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Serrano, Laura Graikini, Dimitra Universidad de Zaragoza Pérez, María Dolores Universidad de Zaragoza (orcid)0000-0003-2555-8425 Grasa, Laura Universidad de Zaragoza (orcid)0000-0002-5306-9365 Sánchez, Lourdes Universidad de Zaragoza (orcid)0000-0001-5964-823X 1012 410 Universidad de Zaragoza Dpto. Farmac.Fisiol.y Med.L.F. Área Fisiología 2008 780 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Tecnología de Alimentos 36, 3 (2023), 667–681 BioMetals BIOMETALS 0966-0844 1408061 http://zaguan.unizar.es/record/120995/files/texto_completo.pdf Versión publicada 1923340 http://zaguan.unizar.es/record/120995/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:120995 articulos driver 2024-11-22-11:57:57 ARTICLE