000121011 001__ 121011 000121011 005__ 20240319081026.0 000121011 0247_ $$2doi$$a10.3389/fvets.2022.957153 000121011 0248_ $$2sideral$$a131026 000121011 037__ $$aART-2022-131026 000121011 041__ $$aeng 000121011 100__ $$0(orcid)0000-0002-1481-9805$$aCequier, Alina$$uUniversidad de Zaragoza 000121011 245__ $$aThe immunomodulation–immunogenicity balance of equine Mesenchymal Stem Cells (MSCs) is differentially affected by the immune cell response depending on inflammatory licensing and major histocompatibility complex (MHC) compatibility 000121011 260__ $$c2022 000121011 5060_ $$aAccess copy available to the general public$$fUnrestricted 000121011 5203_ $$aThe immunomodulatory properties of equine mesenchymal stem cells (MSCs) are important for their therapeutic potential and for their facilitating role in their escape from immune recognition, which may also be influenced by donor–recipient major histocompatibility complex (MHC) matching/mismatching and MHC expression level. Factors such as inflammation can modify the balance between regulatory and immunogenic profiles of equine MSCs, but little is known about how the exposure to the immune system can affect these properties in equine MSCs. In this study, we analyzed the gene expression and secretion of molecules related to the immunomodulation and immunogenicity of equine MSCs, either non-manipulated (MSC-naive) or stimulated by pro-inflammatory cytokines (MSC-primed), before and after their exposure to autologous or allogeneic MHC-matched/-mismatched lymphocytes, either activated or resting. Cytokine priming induced the immunomodulatory profile of MSCs at the baseline (MSCs cultured alone), and the exposure to activated lymphocytes further increased the expression of interleukin 6 (IL6), cyclooxygenase 2, and inducible nitric oxide synthase, and IL6 secretion. Activated lymphocytes were also able to upregulate the regulatory profile of MSC-naive to levels comparable to cytokine priming. On the contrary, resting lymphocytes did not upregulate the immunomodulatory profile of equine MSCs, but interestingly, MSC-primed exposed to MHC-mismatched lymphocytes showed the highest expression and secretion of these mediators, which may be potentially linked to the activation of lymphocytes upon recognition of foreign MHC molecules. Cytokine priming alone did not upregulate the immunogenic genes, but MSC-primed exposed to activated or resting lymphocytes increased their MHC-I and MHC-II expression, regardless of the MHC-compatibility. The upregulation of immunogenic markers including CD40 in the MHC-mismatched co-culture might have activated lymphocytes, which, at the same time, could have promoted the immune regulatory profile aforementioned. In conclusion, activated lymphocytes are able to induce the equine MSC regulatory profile, and their effects seem to be additive to the priming action. Importantly, our results suggest that the lymphocyte response against MHC-mismatched MSC-primed would promote further activation of their immunomodulatory ability, which eventually might help them evade this reaction. Further studies are needed to clarify how these findings might have clinical implications in vivo, which will help developing safer and more effective therapies. 000121011 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A19-17R$$9info:eu-repo/grantAgreement/ES/MICINN/PID2020-116352GB-I00$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2017-84411-P 000121011 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000121011 590__ $$a3.2$$b2022 000121011 592__ $$a0.737$$b2022 000121011 591__ $$aVETERINARY SCIENCES$$b12 / 144 = 0.083$$c2022$$dQ1$$eT1 000121011 593__ $$aVeterinary (miscellaneous)$$c2022$$dQ1 000121011 594__ $$a3.8$$b2022 000121011 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000121011 700__ $$0(orcid)0000-0002-8712-2275$$aVázquez, Francisco José$$uUniversidad de Zaragoza 000121011 700__ $$0(orcid)0000-0001-7188-0461$$aRomero, Antonio$$uUniversidad de Zaragoza 000121011 700__ $$0(orcid)0000-0003-1286-4968$$aVitoria, Arantza$$uUniversidad de Zaragoza 000121011 700__ $$0(orcid)0000-0002-9710-0395$$aBernad, Elvira$$uUniversidad de Zaragoza 000121011 700__ $$0(orcid)0000-0002-8752-5146$$aGarcía-Martínez, Mirta$$uUniversidad de Zaragoza 000121011 700__ $$aGascón, Isabel 000121011 700__ $$0(orcid)0000-0001-9818-508X$$aBarrachina, Laura$$uUniversidad de Zaragoza 000121011 700__ $$0(orcid)0000-0003-3289-2675$$aRodellar, Clementina$$uUniversidad de Zaragoza 000121011 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética 000121011 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal 000121011 773__ $$g9 (2022), 957153 [ 22 pp.]$$pFront. vet. sci.$$tFrontiers in Veterinary Science$$x2297-1769 000121011 8564_ $$s3689935$$uhttps://zaguan.unizar.es/record/121011/files/texto_completo.pdf$$yVersión publicada 000121011 8564_ $$s2318445$$uhttps://zaguan.unizar.es/record/121011/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000121011 909CO $$ooai:zaguan.unizar.es:121011$$particulos$$pdriver 000121011 951__ $$a2024-03-18-16:44:21 000121011 980__ $$aARTICLE