000121025 001__ 121025
000121025 005__ 20240319080956.0
000121025 0247_ $$2doi$$a10.1016/j.rvsc.2022.01.018
000121025 0248_ $$2sideral$$a131524
000121025 037__ $$aART-2022-131524
000121025 041__ $$aeng
000121025 100__ $$0(orcid)0000-0003-2957-1379$$aVerde, María Teresa$$uUniversidad de Zaragoza
000121025 245__ $$aComparison of circulating CD4+, CD8+ lymphocytes and cytokine profiles between dogs with atopic dermatitis and healthy dogs
000121025 260__ $$c2022
000121025 5060_ $$aAccess copy available to the general public$$fUnrestricted
000121025 5203_ $$aIntroduction
Canine atopic dermatitis (cAD) is an inflammatory skin disease characterized by impaired immune function. Changes in the proportions of CD4+ and CD8+ lymphocytes and the serum concentrations of cytokines in the pathogenesis of cAD have been described.
Objectives
To assess whether the changes in the ratio of CD4+ and CD8+ lymphocytes in the peripheral blood of atopic dogs at the time of diagnosis are related to the severity of the disease. Furthermore, we determined whether the changes in the serum concentrations of the cytokines IL-31, IL-34, IL-10, IFN-γ, and TNF-α were different between atopic and control dogs.
Procedures
Fifty-six client-owned dogs with atopic dermatitis and 53 healthy control dogs were used. The percentages of CD4+ and CD8+ lymphocytes were determined by imaging flow cytometry. The index of CADESI-03 was calculated. Serum cytokine levels were analyzed using ELISA.
Results
Atopic dogs showed a higher percentage of CD8+ lymphocytes, a lower CD4+/CD8+ ratio than healthy dogs, and a positive correlation with CADESI-03. Atopic dogs also showed higher serum IL-31 and IL-34 levels and lower IL-10 levels. A moderate positive correlation was found between serum IL-31 and CADESI-03.
Conclusions
The CD4+/CD8+ ratio may be a sensitive parameter that positively correlates with the severity of cAD, and elevated serum levels of IL-31 and IL-34 may facilitate diagnosis of the disease.
000121025 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000121025 590__ $$a2.4$$b2022
000121025 592__ $$a0.577$$b2022
000121025 591__ $$aVETERINARY SCIENCES$$b29 / 144 = 0.201$$c2022$$dQ1$$eT1
000121025 593__ $$aVeterinary (miscellaneous)$$c2022$$dQ1
000121025 594__ $$a4.1$$b2022
000121025 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000121025 700__ $$0(orcid)0000-0001-6209-4282$$aVillanueva-Saz, Sergio$$uUniversidad de Zaragoza
000121025 700__ $$0(orcid)0000-0002-1934-5397$$aLoste, Araceli$$uUniversidad de Zaragoza
000121025 700__ $$aMarteles, Diana
000121025 700__ $$0(orcid)0000-0002-9357-143X$$aPereboom, Desirée$$uUniversidad de Zaragoza
000121025 700__ $$0(orcid)0000-0002-9500-4703$$aConde, Tomás$$uUniversidad de Zaragoza
000121025 700__ $$aFernández, Antonio
000121025 7102_ $$11012$$2410$$aUniversidad de Zaragoza$$bDpto. Farmac.Fisiol.y Med.L.F.$$cÁrea Fisiología
000121025 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000121025 773__ $$g145 (2022), 13-20$$pRes. Vet. Sci.$$tRESEARCH IN VETERINARY SCIENCE$$x0034-5288
000121025 8564_ $$s1409532$$uhttps://zaguan.unizar.es/record/121025/files/texto_completo.pdf$$yVersión publicada
000121025 8564_ $$s2534602$$uhttps://zaguan.unizar.es/record/121025/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000121025 909CO $$ooai:zaguan.unizar.es:121025$$particulos$$pdriver
000121025 951__ $$a2024-03-18-13:41:00
000121025 980__ $$aARTICLE