000121028 001__ 121028 000121028 005__ 20240319081000.0 000121028 0247_ $$2doi$$a10.1371/journal.pone.0275375 000121028 0248_ $$2sideral$$a131478 000121028 037__ $$aART-2022-131478 000121028 041__ $$aeng 000121028 100__ $$aNess, Anthony 000121028 245__ $$aChronic wasting disease prions in mule deer interdigital glands 000121028 260__ $$c2022 000121028 5060_ $$aAccess copy available to the general public$$fUnrestricted 000121028 5203_ $$aChronic wasting disease (CWD) is a geographically expanding, fatal neurodegenerative disease in cervids. The disease can be transmitted directly (animal-animal) or indirectly via infectious prions shed into the environment. The precise mechanisms of indirect CWD transmission are unclear but known sources of the infectious prions that contaminate the environment include saliva, urine and feces. We have previously identified PrPC expression in deer interdigital glands, sac-like exocrine structures located between the digits of the hooves. In this study, we assayed for CWD prions within the interdigital glands of CWD infected deer to determine if they could serve as a source of prion shedding and potentially contribute to CWD transmission. Immunohistochemical analysis of interdigital glands from a CWD-infected female mule deer identified disease-associated PrPCWD within clusters of infiltrating leukocytes adjacent to sudoriferous and sebaceous glands, and within the acrosyringeal epidermis of a sudoriferous gland tubule. Proteinase K-resistant PrPCWD material was amplified by serial protein misfolding cyclic amplification (sPMCA) from soil retrieved from between the hoof digits of a clinically affected mule deer. Blinded testing of interdigital glands from 11 mule deer by real-time quake-induced conversion (RT-QuIC) accurately identified CWD-infected animals. The data described suggests that interdigital glands may play a role in the dissemination of CWD prions into the environment, warranting future investigation. 000121028 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000121028 590__ $$a3.7$$b2022 000121028 592__ $$a0.885$$b2022 000121028 591__ $$aMULTIDISCIPLINARY SCIENCES$$b26 / 73 = 0.356$$c2022$$dQ2$$eT2 000121028 593__ $$aMultidisciplinary$$c2022$$dQ1 000121028 594__ $$a6.0$$b2022 000121028 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000121028 700__ $$aZeng, Doris 000121028 700__ $$aKuznetsova, Alsu 000121028 700__ $$aOtero, Alicia 000121028 700__ $$aKim, Chiye 000121028 700__ $$aSaboraki, Kelsey 000121028 700__ $$aLingle, Susan 000121028 700__ $$aPybus, Margo 000121028 700__ $$aAiken, Judd 000121028 700__ $$aGilch, Sabine 000121028 700__ $$aMcKenzie, Debbie 000121028 773__ $$g17, 10 (2022), e0275375 [18 pp.]$$pPLoS One$$tPLoS ONE$$x1932-6203 000121028 8564_ $$s6524881$$uhttps://zaguan.unizar.es/record/121028/files/texto_completo.pdf$$yVersión publicada 000121028 8564_ $$s2344510$$uhttps://zaguan.unizar.es/record/121028/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000121028 909CO $$ooai:zaguan.unizar.es:121028$$particulos$$pdriver 000121028 951__ $$a2024-03-18-14:07:40 000121028 980__ $$aARTICLE