121170 20230519145626.0 doi 10.3390/antiox10091446 sideral 131282 ART-2021-131282 eng Sánchez-Ruiz, María Isabel Agaricales mushroom lignin peroxidase: from structure–function to degradative capabilities 2021 Access copy available to the general public Unrestricted Lignin biodegradation has been extensively studied in white-rot fungi, which largely belong to order Polyporales. Among the enzymes that wood-rotting polypores secrete, lignin peroxidases (LiPs) have been labeled as the most efficient. Here, we characterize a similar enzyme (ApeLiP) from a fungus of the order Agaricales (with ~13,000 described species), the soil-inhabiting mushroom Agrocybe pediades. X-ray crystallography revealed that ApeLiP is structurally related to Polyporales LiPs, with a conserved heme-pocket and a solvent-exposed tryptophan. Its biochemical characterization shows that ApeLiP can oxidize both phenolic and non-phenolic lignin model-compounds, as well as different dyes. Moreover, using stopped-flow rapid spectrophotometry and 2D-NMR, we demonstrate that ApeLiP can also act on real lignin. Characterization of a variant lacking the above tryptophan residue shows that this is the oxidation site for lignin and other high redox-potential substrates, and also plays a role in phenolic substrate oxidation. The reduction potentials of the catalytic-cycle intermediates were estimated by stopped-flow in equilibrium reactions, showing similar activation by H2O2, but a lower potential for the rate-limiting step (compound-II reduction) compared to other LiPs. Unexpectedly, ApeLiP was stable from acidic to basic pH, a relevant feature for application considering its different optima for oxidation of phenolic and nonphenolic compounds. info:eu-repo/grantAgreement/ES/CSIC/PIE-202120E019 info:eu-repo/grantAgreement/ES/MCIN/FEDER/BIO2017-86559-R info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 7.675 2021 BIOCHEMISTRY & MOLECULAR BIOLOGY 50 / 297 = 0.168 2021 Q1 T1 FOOD SCIENCE & TECHNOLOGY 12 / 144 = 0.083 2021 Q1 T1 CHEMISTRY, MEDICINAL 4 / 63 = 0.063 2021 Q1 T1 1.008 2021 Biochemistry 2021 Q1 Physiology 2021 Q1 Molecular Biology 2021 Q1 Clinical Biochemistry 2021 Q1 6.5 2021 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Ayuso Fernández, Iván Rencoret, Jorge González Ramírez, Andrés Manuel Universidad de Zaragoza (orcid)0000-0002-5838-0857 Linde, Dolores Davó Siguero, Irene Romero, Antonio Gutiérrez, Ana Martínez, Angel T. Ruiz Dueñas, Francisco Javier 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 10, 9 (2021), 1446 [23 pp] Antioxidants Antioxidants 2076-3921 3175809 http://zaguan.unizar.es/record/121170/files/texto_completo.pdf Versión publicada 2834771 http://zaguan.unizar.es/record/121170/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:121170 articulos driver 2023-05-18-16:18:57 ARTICLE