000121176 001__ 121176
000121176 005__ 20250214141227.0
000121176 0247_ $$2doi$$a10.1186/s13567-022-01034-z
000121176 0248_ $$2sideral$$a131824
000121176 037__ $$aART-2022-131824
000121176 041__ $$aeng
000121176 100__ $$0(orcid)0000-0002-7970-4929$$aMuñoz, Pilar M.
000121176 245__ $$aA Brucella melitensis H38¿wbkF rough mutant protects against Brucella ovis in rams
000121176 260__ $$c2022
000121176 5060_ $$aAccess copy available to the general public$$fUnrestricted
000121176 5203_ $$aBrucella melitensis and Brucella ovis are gram-negative pathogens of sheep that cause severe economic losses and, although B. ovis is non-zoonotic, B. melitensis is the main cause of human brucellosis. B. melitensis carries a smooth (S) lipopolysaccharide (LPS) with an N-formyl-perosamine O-polysaccharide (O-PS) that is absent in the rough LPS of B. ovis. Their control and eradication require vaccination, but B. melitensis Rev 1, the only vaccine available, triggers anti-O-PS antibodies that interfere in the S-brucellae serodiagnosis. Since eradication and serological surveillance of the zoonotic species are priorities, Rev 1 is banned once B. melitensis is eradicated or where it never existed, hampering B. ovis control and eradication. To develop a B. ovis specific vaccine, we investigated three Brucella live vaccine candidates lacking N-formyl-perosamine O-PS: Bov::CAΔwadB (CO2-independent B. ovis with truncated LPS core oligosaccharide); Rev1::wbdRΔwbkC (carrying N-acetylated O-PS); and H38ΔwbkF (B. melitensis rough mutant with intact LPS core). After confirming their attenuation and protection against B. ovis in mice, were tested in rams for efficacy. H38ΔwbkF yielded similar protection to Rev 1 against B. ovis but Bov::CAΔwadB and Rev1::wbdRΔwbkC conferred no or poor protection, respectively. All H38ΔwbkF vaccinated rams developed a protracted antibody response in ELISA and immunoprecipitation B. ovis diagnostic tests. In contrast, all remained negative in Rose Bengal and complement fixation tests used routinely for B. melitensis diagnosis, though some became positive in S-LPS ELISA owing to LPS core epitope reactivity. Thus, H38ΔwbkF is an interesting candidate for the immunoprophylaxis of B. ovis in B. melitensis-free areas.
000121176 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/BES-2015-075609$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2014-58795-C4-4-R$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2014-58795-C4-3-R$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2014-58795-C4-1-R$$9info:eu-repo/grantAgreement/ES/MICINN/PID2019-107601RB-C31$$9info:eu-repo/grantAgreement/ES/DGA/A21-20R$$9info:eu-repo/grantAgreement/ES/DGA/A13-17R
000121176 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000121176 590__ $$a4.4$$b2022
000121176 592__ $$a0.869$$b2022
000121176 591__ $$aVETERINARY SCIENCES$$b6 / 144 = 0.042$$c2022$$dQ1$$eT1
000121176 593__ $$aVeterinary (miscellaneous)$$c2022$$dQ1
000121176 594__ $$a6.4$$b2022
000121176 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000121176 700__ $$aConde-Álvarez, Raquel
000121176 700__ $$0(orcid)0000-0002-4127-234X$$aAndrés-Barranco, Sara$$uUniversidad de Zaragoza
000121176 700__ $$ade Miguel, María-Jesús
000121176 700__ $$aZúñiga-Ripa, Amaia
000121176 700__ $$aAragón-Aranda, Beatriz
000121176 700__ $$aSalvador-Bescós, Miriam
000121176 700__ $$aMartínez-Gómez, Estrella
000121176 700__ $$aIriarte, Maite
000121176 700__ $$0(orcid)0000-0002-5225-661X$$aBarberán, Montserrat$$uUniversidad de Zaragoza
000121176 700__ $$aVizcaíno, Nieves
000121176 700__ $$aMoriyón, Ignacio
000121176 700__ $$aBlasco, José M.
000121176 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000121176 773__ $$g53 (2022), [13 pp.]$$pVet. res.$$tVeterinary Research$$x0928-4249
000121176 8564_ $$s1589136$$uhttps://zaguan.unizar.es/record/121176/files/texto_completo.pdf$$yVersión publicada
000121176 8564_ $$s2714635$$uhttps://zaguan.unizar.es/record/121176/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000121176 909CO $$ooai:zaguan.unizar.es:121176$$particulos$$pdriver
000121176 951__ $$a2025-02-14-14:11:20
000121176 980__ $$aARTICLE