000121190 001__ 121190
000121190 005__ 20241125101127.0
000121190 0247_ $$2doi$$a10.1016/j.theriogenology.2022.12.022
000121190 0248_ $$2sideral$$a131715
000121190 037__ $$aART-2023-131715
000121190 041__ $$aeng
000121190 100__ $$0(orcid)0000-0001-6733-9373$$aMiguel-Jiménez, Sara
000121190 245__ $$aIn vitro approach points to a chemotactic effect of melatonin on ram spermatozoa
000121190 260__ $$c2023
000121190 5060_ $$aAccess copy available to the general public$$fUnrestricted
000121190 5203_ $$aSperm orientation mechanisms, such as chemotaxis, are essential for the sperm to reach the oocyte and fertilize it. Melatonin is secreted by the cumulus cells and is also present in the follicular fluid in mammals. The presence of membrane receptors for melatonin in ram spermatozoa, and its proven involvement in the sperm functionality, may suggest a possible role in the guided movement towards the oocyte. Hence, the objective of the present work is to study the in vitro potential chemotactic action of melatonin on ram spermatozoa, analysing the influence of the season (breeding and non-breeding) and the sperm capacitation state. The first experimental approach consisted in the inclusion of melatonin in the upper layer of a swim-up selection method. During the non-breeding season, the presence of melatonin at 100 pM and 1 μM concentrations significantly increased the cell recovery rate, and induced changes in the sperm location of the MT2 melatonin receptor, compared with the standard swim-up. Moreover, the selected sperm population with 100 pM melatonin presented a higher percentage of capacitated spermatozoa. The greater recovery rate obtained with melatonin could be due to the stimulation of sperm movement in random directions, i.e., a chemokinetic effect, or due to a guided movement (chemotaxis) towards the gradient of the melatonin. To elucidate this issue, together with the study of the influence of the sperm capacitation status, we performed a second experimental approach which consisted in the use of chemotaxis chambers and an open-source software (Open-CASA) that analyses the sperm trajectories towards the hormone gradient and calculates a chemotaxis index (SL index). There was a significant difference between the SL index in the presence of 1 μM melatonin and the control without hormone. This effect was only observed in capacitated spermatozoa with cAMP-elevating agents (Cap-CK samples) obtained during the non-breeding season. These results would point to an in vitro chemotactic effect of melatonin on ram spermatozoa, although chemokinesis cannot be ruled out. Nonetheless, the inclusion of this hormone in the swim-up procedure could enhance the sperm recovery rate.
000121190 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A07-20R$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2017-83799-R
000121190 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000121190 590__ $$a2.4$$b2023
000121190 592__ $$a0.738$$b2023
000121190 591__ $$aVETERINARY SCIENCES$$b24 / 167 = 0.144$$c2023$$dQ1$$eT1
000121190 593__ $$aAnimal Science and Zoology$$c2023$$dQ1
000121190 591__ $$aREPRODUCTIVE BIOLOGY$$b22 / 39 = 0.564$$c2023$$dQ3$$eT2
000121190 593__ $$aSmall Animals$$c2023$$dQ1
000121190 593__ $$aFood Animals$$c2023$$dQ1
000121190 593__ $$aEquine$$c2023$$dQ1
000121190 594__ $$a5.5$$b2023
000121190 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000121190 700__ $$aBorao, Sonia
000121190 700__ $$aPortolés-Bayod, Virginia
000121190 700__ $$0(orcid)0000-0003-1997-4262$$aCasao, Adriana$$uUniversidad de Zaragoza
000121190 700__ $$0(orcid)0000-0002-2312-6402$$aPérez-Pe, Rosaura$$uUniversidad de Zaragoza
000121190 7102_ $$11002$$2819$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Zoología
000121190 773__ $$g198 (2023), 36-46$$p1879-3231]$$tTHERIOGENOLOGY$$x0093-691X
000121190 8564_ $$s1995747$$uhttps://zaguan.unizar.es/record/121190/files/texto_completo.pdf$$yVersión publicada
000121190 8564_ $$s2521369$$uhttps://zaguan.unizar.es/record/121190/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000121190 909CO $$ooai:zaguan.unizar.es:121190$$particulos$$pdriver
000121190 951__ $$a2024-11-22-11:57:50
000121190 980__ $$aARTICLE