000121220 001__ 121220
000121220 005__ 20240319081022.0
000121220 0247_ $$2doi$$a10.1038/s41598-022-23343-1
000121220 0248_ $$2sideral$$a131728
000121220 037__ $$aART-2022-131728
000121220 041__ $$aeng
000121220 100__ $$aComín, Jessica
000121220 245__ $$aAnalysis of the twenty-six largest outbreaks of tuberculosis in Aragon using whole-genome sequencing for surveillance purposes
000121220 260__ $$c2022
000121220 5060_ $$aAccess copy available to the general public$$fUnrestricted
000121220 5203_ $$aThe incidence of tuberculosis in Aragon, Spain, is around ten cases per 100,000 inhabitants. Since 2004, a molecular surveillance protocol has been carried out; therefore, all M. tuberculosis strains are genotyped. Recently, whole-genome sequencing has been implemented for relevant isolates. The aim of this work is to characterise at the molecular level the causative strains of the 26 largest outbreaks of the community (including ten or more cases), genotyped by IS6110-RFLP and causing 26% of tuberculosis cases. To achieve this objective, two or three isolates of each IS6110-cluster belonging to different years were selected for sequencing. We found that strains of lineages L4.8, L4.3 and L4.1.2 were the most frequent. The threshold of 12 SNPs as the maximum distance for confirming the belonging to an outbreak was met for 18 of the 26 IS6110-clusters. Four pairs of isolates with more than 90 SNPs were identified as not belonging to the same strain, and four other pairs were kept in doubt as the number of SNPs was close to 12, between 14 and 35. The study of Regions of Difference revealed that they are lineage conserved. Moreover, we could analyse the IS6110 locations for all genome-sequenced isolates, finding some frequent locations in isolates belonging to the same lineage and certain IS6110 movements between the paired isolates. In the vast majority, these movements were not captured by the IS6110-RFLP pattern. After classifying the genes containing SNP by their functional category, we could confirm that the number of SNPs detected in genes considered as virulence factors and the number of cases the strain produced were not related, suggesting that a particular SNP is more relevant than the number. The characteristics found in the most successful strains in our community could be useful for other researchers in epidemiology, virulence and pathogenesis.
000121220 536__ $$9info:eu-repo/grantAgreement/ES/ISCIII/FIS18-0336
000121220 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000121220 590__ $$a4.6$$b2022
000121220 592__ $$a0.973$$b2022
000121220 591__ $$aMULTIDISCIPLINARY SCIENCES$$b22 / 73 = 0.301$$c2022$$dQ2$$eT1
000121220 593__ $$aMultidisciplinary$$c2022$$dQ1
000121220 594__ $$a7.5$$b2022
000121220 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000121220 700__ $$0(orcid)0000-0002-3312-7759$$aCebollada, Alberto
000121220 700__ $$aIbarz, Daniel$$uUniversidad de Zaragoza
000121220 700__ $$0(orcid)0000-0003-2044-6782$$aViñuelas, Jesús$$uUniversidad de Zaragoza
000121220 700__ $$aSahagún, Juan$$uUniversidad de Zaragoza
000121220 700__ $$aTorres, Luis
000121220 700__ $$0(orcid)0000-0003-2266-8602$$aIglesias, María-José$$uUniversidad de Zaragoza
000121220 700__ $$0(orcid)0000-0002-3011-2519$$aSamper, Sofía$$uUniversidad de Zaragoza
000121220 7102_ $$11011$$2630$$aUniversidad de Zaragoza$$bDpto. Microb.Ped.Radio.Sal.Pú.$$cÁrea Microbiología
000121220 7102_ $$11011$$2615$$aUniversidad de Zaragoza$$bDpto. Microb.Ped.Radio.Sal.Pú.$$cÁrea Medic.Prevent.Salud Públ.
000121220 773__ $$g12 (2022), 18766 [10 pp.]$$pSci. rep. (Nat. Publ. Group)$$tScientific reports (Nature Publishing Group)$$x2045-2322
000121220 8564_ $$s1560836$$uhttps://zaguan.unizar.es/record/121220/files/texto_completo.pdf$$yVersión publicada
000121220 8564_ $$s2481022$$uhttps://zaguan.unizar.es/record/121220/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000121220 909CO $$ooai:zaguan.unizar.es:121220$$particulos$$pdriver
000121220 951__ $$a2024-03-18-16:19:38
000121220 980__ $$aARTICLE