121330 20230519145624.0 doi 10.3390/polym13193399 sideral 131314 ART-2021-131314 eng Ren, Jiongyu Poly-e-Caprolactone/Fibrin-Alginate scaffold: a new Pro-Angiogenic composite biomaterial for the treatment of bone defects 2021 Access copy available to the general public Unrestricted We hypothesized that a composite of 3D porous melt-electrowritten poly-ɛ-caprolactone (PCL) coated throughout with a porous and slowly biodegradable fibrin/alginate (FA) matrix would accelerate bone repair due to its angiogenic potential. Scanning electron microscopy showed that the open pore structure of the FA matrix was maintained in the PCL/FA composites. Fourier transform infrared spectroscopy and differential scanning calorimetry showed complete coverage of the PCL fibres by FA, and the PCL/FA crystallinity was decreased compared with PCL. In vitro cell work with osteoprogenitor cells showed that they preferentially bound to the FA component and proliferated on all scaffolds over 28 days. A chorioallantoic membrane assay showed more blood vessel infiltration into FA and PCL/FA compared with PCL, and a significantly higher number of bifurcation points for PCL/FA compared with both FA and PCL. Implantation into a rat cranial defect model followed by microcomputed tomography, histology, and immunohistochemistry after 4- and 12-weeks post operation showed fast early bone formation at week 4, with significantly higher bone formation for FA and PCL/FA compared with PCL. However, this phenomenon was not extrapolated to week 12. Therefore, for long-term bone regeneration, tuning of FA degradation to ensure syncing with new bone formation is likely necessary. info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 4.967 2021 POLYMER SCIENCE 16 / 90 = 0.178 2021 Q1 T1 0.726 2021 Polymers and Plastics 2021 Q1 Chemistry (miscellaneous) 2021 Q1 5.7 2021 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Kohli, Nupur Sharma, Vaibhav Shakouri, Taleen Keskin-Erdogan, Zalike Saifzadeh, Siamak Brierly, Gary I. Knowles, Jonathan C. Woodruff, Maria A. García-Gareta, Elena (orcid)0000-0001-7062-9099 13, 19 (2021), 3399 [22 pp.] Polymers (Basel) Polymers 2073-4360 11551393 http://zaguan.unizar.es/record/121330/files/texto_completo.pdf Versión publicada 2808675 http://zaguan.unizar.es/record/121330/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:121330 articulos driver 2023-05-18-16:18:16 ARTICLE