doi:10.1038/s41598-022-04971-zengHerrera-Marcos, Luis V.Martínez-Beamonte, RobertoMacías-Herranz, ManuelArnal, CarmenBarranquero, CristinaPuente-Lanzarote, Juan J.Gascón, SoniaHerrero-Continente, TaniaGonzalo-Romeo, GonzaloAlastrué-Vera, VíctorGutiérrez-Blázquez, DoloresLou-Bonafonte, José M.Surra, Joaquín C.Rodríguez-Yoldi, María J.García-Gil, AgustínGüemes, AntonioOsada, JesúsHepatic galectin-3 is associated with lipid droplet area in non-alcoholic steatohepatitis in a new swine modelART-2022-131928Non-alcoholic fatty liver disease (NAFLD) is currently a growing epidemic disease that can lead to cirrhosis and hepatic cancer when it evolves into non-alcoholic steatohepatitis (NASH), a gap not well understood. To characterize this disease, pigs, considered to be one of the most similar to human experimental animal models, were used. To date, all swine-based settings have been carried out using rare predisposed breeds or long-term experiments. Herein, we fully describe a new experimental swine model for initial and reversible NASH using cross-bred animals fed on a high saturated fat, fructose, cholesterol, cholate, choline and methionine-deficient diet. To gain insight into the hepatic transcriptome that undergoes steatosis and steatohepatitis, we used RNA sequencing. This process significantly up-regulated 976 and down-regulated 209 genes mainly involved in cellular processes. Gene expression changes of 22 selected transcripts were verified by RT-qPCR. Lipid droplet area was positively associated with CD68, GPNMB, LGALS3, SLC51B and SPP1, and negatively with SQLE expressions. When these genes were tested in a second experiment of NASH reversion, LGALS3, SLC51B and SPP1 significantly decreased their expression. However, only LGALS3 was associated with lipid droplet areas. Our results suggest a role for LGALS3 in the transition of NAFLD to NASH.2022http://zaguan.unizar.es/record/12140110.1038/s41598-022-04971-zhttp://zaguan.unizar.es/record/121401oai:zaguan.unizar.es:121401info:eu-repo/grantAgreement/ES/DGA/B16-20Rinfo:eu-repo/grantAgreement/ES/ISCIII-CIBERObn/CB06-03-1012info:eu-repo/grantAgreement/ES/MICINN-ISCIIII-FEDER/PID2019-104915RB-I00info:eu-repo/grantAgreement/ES/MINECO/SAF2016-75441-RScientific reports (Nature Publishing Group) 12 (2022), 1024 [17 pp.]byhttp://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccess