121401 20240705134139.0 doi 10.1038/s41598-022-04971-z sideral 131928 ART-2022-131928 eng Herrera-Marcos, Luis V. Universidad de Zaragoza (orcid)0000-0002-4665-9674 Hepatic galectin-3 is associated with lipid droplet area in non-alcoholic steatohepatitis in a new swine model 2022 Access copy available to the general public Unrestricted Non-alcoholic fatty liver disease (NAFLD) is currently a growing epidemic disease that can lead to cirrhosis and hepatic cancer when it evolves into non-alcoholic steatohepatitis (NASH), a gap not well understood. To characterize this disease, pigs, considered to be one of the most similar to human experimental animal models, were used. To date, all swine-based settings have been carried out using rare predisposed breeds or long-term experiments. Herein, we fully describe a new experimental swine model for initial and reversible NASH using cross-bred animals fed on a high saturated fat, fructose, cholesterol, cholate, choline and methionine-deficient diet. To gain insight into the hepatic transcriptome that undergoes steatosis and steatohepatitis, we used RNA sequencing. This process significantly up-regulated 976 and down-regulated 209 genes mainly involved in cellular processes. Gene expression changes of 22 selected transcripts were verified by RT-qPCR. Lipid droplet area was positively associated with CD68, GPNMB, LGALS3, SLC51B and SPP1, and negatively with SQLE expressions. When these genes were tested in a second experiment of NASH reversion, LGALS3, SLC51B and SPP1 significantly decreased their expression. However, only LGALS3 was associated with lipid droplet areas. Our results suggest a role for LGALS3 in the transition of NAFLD to NASH. info:eu-repo/grantAgreement/ES/DGA/B16-20R info:eu-repo/grantAgreement/ES/ISCIII-CIBERObn/CB06-03-1012 info:eu-repo/grantAgreement/ES/MICINN-ISCIIII-FEDER/PID2019-104915RB-I00 info:eu-repo/grantAgreement/ES/MINECO/SAF2016-75441-R info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 4.6 2022 MULTIDISCIPLINARY SCIENCES 22 / 73 = 0.301 2022 Q2 T1 0.973 2022 Multidisciplinary 2022 Q1 7.5 2022 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Martínez-Beamonte, Roberto Universidad de Zaragoza (orcid)0000-0002-8100-5596 Macías-Herranz, Manuel Arnal, Carmen Universidad de Zaragoza (orcid)0000-0003-1197-4276 Barranquero, Cristina (orcid)0000-0002-8442-8041 Puente-Lanzarote, Juan J. Universidad de Zaragoza (orcid)0000-0002-7213-7095 Gascón, Sonia Universidad de Zaragoza Herrero-Continente, Tania Gonzalo-Romeo, Gonzalo Universidad de Zaragoza (orcid)0009-0008-6478-7857 Alastrué-Vera, Víctor Gutiérrez-Blázquez, Dolores Lou-Bonafonte, José M. Universidad de Zaragoza (orcid)0000-0001-9578-6525 Surra, Joaquín C. Universidad de Zaragoza (orcid)0000-0002-5841-0462 Rodríguez-Yoldi, María J. Universidad de Zaragoza (orcid)0000-0002-3595-7668 García-Gil, Agustín (orcid)0000-0002-8275-7191 Güemes, Antonio Universidad de Zaragoza (orcid)0000-0002-4437-2581 Osada, Jesús Universidad de Zaragoza (orcid)0000-0002-8251-8457 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 1012 410 Universidad de Zaragoza Dpto. Farmac.Fisiol.y Med.L.F. Área Fisiología 2008 700 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Producción Animal 1013 090 Universidad de Zaragoza Dpto. Cirugía Área Cirugía 1009 X Universidad de Zaragoza Dpto. Patología Animal Área Técnica. Lab. y Talleres 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 1002 050 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Biología Celular 12 (2022), 1024 [17 pp.] Sci. rep. (Nat. Publ. Group) Scientific reports (Nature Publishing Group) 2045-2322 9999940 http://zaguan.unizar.es/record/121401/files/texto_completo.pdf Versión publicada 2560459 http://zaguan.unizar.es/record/121401/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:121401 articulos driver 2024-07-05-12:46:14 ARTICLE