000121409 001__ 121409
000121409 005__ 20230519145628.0
000121409 0247_ $$2doi$$a10.2478/jvetres-2021-0052
000121409 0248_ $$2sideral$$a131966
000121409 037__ $$aART-2021-131966
000121409 041__ $$aeng
000121409 100__ $$0(orcid)0000-0003-1417-272X$$aGracia Salinas, María Jesús$$uUniversidad de Zaragoza
000121409 245__ $$aPrevalence of Toxoplasma gondii in retail fresh meat products from free-range chickens in Spain
000121409 260__ $$c2021
000121409 5060_ $$aAccess copy available to the general public$$fUnrestricted
000121409 5203_ $$aIntroduction: Toxoplasma gondii is one of the most prevalent zoonotic protozoan parasites worldwide and affects the vast majority of warm-blooded animal species, including humans. Postnatal infection in humans occurs through the ingestion of sporulated T. gondii oocysts or via the oral intake of parasite tissue cysts during the consumption of raw or undercooked meat. In this regard, given their high exposure to oocysts, chickens (Gallus domesticus) raised on the ground constitute a potential source of T. gondii. Material and Methods: For the first time in Spain, a survey was undertaken in commercial retail free-range poultry. A total of 50 thighs from different animals were analysed. The samples were homogenised and an acid pepsin digestion procedure was applied prior to molecular analysis. Toxoplasma gondii DNA was isolated from meat by qPCR. Two sets of primers were used for DNA amplification targeting the specific sequence of a 529 bp repeat element and another set of primers was utilised for the surface antigen protein-1 gene. Results: DNA extracted from 5 out of 50 tissue samples was positive for both genes by qPCR amplification. Conclusion: The 10% prevalence of Toxoplasma infection found in commercial free-range chickens raises public health issues.
000121409 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A17-17R
000121409 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000121409 590__ $$a2.058$$b2021
000121409 591__ $$aVETERINARY SCIENCES$$b48 / 145 = 0.331$$c2021$$dQ2$$eT2
000121409 592__ $$a0.431$$b2021
000121409 593__ $$aVeterinary (miscellaneous)$$c2021$$dQ2
000121409 594__ $$a3.4$$b2021
000121409 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000121409 700__ $$aEscolano Campos, Cristina
000121409 700__ $$0(orcid)0000-0001-5750-3643$$aPeris Peris, María Paz$$uUniversidad de Zaragoza
000121409 700__ $$0(orcid)0000-0002-4121-8087$$aHalaihel Kassab, Nabil$$uUniversidad de Zaragoza
000121409 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000121409 773__ $$g65, 4 (2021), 457 - 461$$pJ. Vet. Res.$$tJournal of Veterinary Research$$x2450-7393
000121409 8564_ $$s350021$$uhttps://zaguan.unizar.es/record/121409/files/texto_completo.pdf$$yVersión publicada
000121409 8564_ $$s2476914$$uhttps://zaguan.unizar.es/record/121409/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000121409 909CO $$ooai:zaguan.unizar.es:121409$$particulos$$pdriver
000121409 951__ $$a2023-05-18-16:19:53
000121409 980__ $$aARTICLE