121409 20230519145628.0 doi 10.2478/jvetres-2021-0052 sideral 131966 ART-2021-131966 eng Gracia Salinas, María Jesús Universidad de Zaragoza (orcid)0000-0003-1417-272X Prevalence of Toxoplasma gondii in retail fresh meat products from free-range chickens in Spain 2021 Access copy available to the general public Unrestricted Introduction: Toxoplasma gondii is one of the most prevalent zoonotic protozoan parasites worldwide and affects the vast majority of warm-blooded animal species, including humans. Postnatal infection in humans occurs through the ingestion of sporulated T. gondii oocysts or via the oral intake of parasite tissue cysts during the consumption of raw or undercooked meat. In this regard, given their high exposure to oocysts, chickens (Gallus domesticus) raised on the ground constitute a potential source of T. gondii. Material and Methods: For the first time in Spain, a survey was undertaken in commercial retail free-range poultry. A total of 50 thighs from different animals were analysed. The samples were homogenised and an acid pepsin digestion procedure was applied prior to molecular analysis. Toxoplasma gondii DNA was isolated from meat by qPCR. Two sets of primers were used for DNA amplification targeting the specific sequence of a 529 bp repeat element and another set of primers was utilised for the surface antigen protein-1 gene. Results: DNA extracted from 5 out of 50 tissue samples was positive for both genes by qPCR amplification. Conclusion: The 10% prevalence of Toxoplasma infection found in commercial free-range chickens raises public health issues. info:eu-repo/grantAgreement/ES/DGA/A17-17R info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 2.058 2021 VETERINARY SCIENCES 48 / 145 = 0.331 2021 Q2 T2 0.431 2021 Veterinary (miscellaneous) 2021 Q2 3.4 2021 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Escolano Campos, Cristina Peris Peris, María Paz Universidad de Zaragoza (orcid)0000-0001-5750-3643 Halaihel Kassab, Nabil Universidad de Zaragoza (orcid)0000-0002-4121-8087 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 65, 4 (2021), 457 - 461 J. Vet. Res. Journal of Veterinary Research 2450-7393 350021 http://zaguan.unizar.es/record/121409/files/texto_completo.pdf Versión publicada 2476914 http://zaguan.unizar.es/record/121409/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:121409 articulos driver 2023-05-18-16:19:53 ARTICLE