121846 20241125101125.0 doi 10.3390/ijms24021154 sideral 132227 ART-2023-132227 eng Sánchez-Hernández, Eva Phytochemical Profiling of Sambucus nigra L. Flower and leaf extracts and their Antimicrobial potential against almond tree pathogens 2023 Access copy available to the general public Unrestricted Despite extensive research on the chemical composition of elderberries and their numerous uses in pharmaceutical, beverage, and food production, there is still a lack of knowledge about Sambucus nigra leaves and flowers’ antimicrobial activity against plant pathogens. In this study, the phytoconstituents of their aqueous ammonia extracts were first characterized by infrared spectroscopy and gas chromatography–mass spectrometry. The major phytocompounds identified in the flower extract were octyl 2-methylpropanoate; 3,5-dihydroxy-6-methyl-2,3-dihydropyran-4-one; propyl malonic acid; adenine; and 1-methyl-2-piperidinemethanol. Concerning the leaf extract, 1,6-anhydro-β-D-glucopyranose; oleic acid; 2,1,3-benzothiadiazole; 2,3-dihydro-benzofuran; and 4-((1E)-3-hydroxy-1-propenyl)-2-methoxyphenol and other phenol derivatives were the main constituents. The potential of the extracts to act as bioprotectants was then investigated against three almond tree pathogens: Diaporthe amygdali, Phytophthora megasperma, and Verticillium dahliae. In vitro tests showed higher activity of the flower extract, with EC90 values in the 241–984 μg·mL−1 range (depending on the pathogen) vs. 354–1322 μg·mL−1 for the leaf extract. In addition, the flower extract led to full protection against P. megasperma at a dose of 1875 μg·mL−1 in ex situ tests on artificially-infected excised almond stems. These inhibitory concentrations were lower than those of commercial fungicides. These findings suggest that S. nigra aerial organs may be susceptible to valorization as an alternative to synthetic fungicides for the protection of this important crop. info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 4.9 2023 BIOCHEMISTRY & MOLECULAR BIOLOGY 66 / 313 = 0.211 2023 Q1 T1 CHEMISTRY, MULTIDISCIPLINARY 68 / 231 = 0.294 2023 Q2 T1 1.179 2023 Medicine (miscellaneous) 2023 Q1 Physical and Theoretical Chemistry 2023 Q1 Computer Science Applications 2023 Q1 Inorganic Chemistry 2023 Q1 Spectroscopy 2023 Q1 Organic Chemistry 2023 Q1 Molecular Biology 2023 Q2 Catalysis 2023 Q2 8.1 2023 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Balduque-Gil, Joaquín (orcid)0000-0001-5136-8639 González-García, Vicente Universidad de Zaragoza (orcid)0000-0002-2535-6914 Barriuso-Vargas, Juan J. Universidad de Zaragoza (orcid)0000-0003-2980-5454 Casanova-Gascón, José Universidad de Zaragoza (orcid)0000-0003-3009-0935 Martín-Gil, Jesús Martín-Ramos, Pablo (orcid)0000-0003-2713-2786 5011 705 Universidad de Zaragoza Dpto. CC.Agrar.y Medio Natural Área Producción Vegetal 5011 063 Universidad de Zaragoza Dpto. CC.Agrar.y Medio Natural Área Botánica 24, 2 (2023), 1154 [17 pp.] Int. j. mol. sci. International Journal of Molecular Sciences 1661-6596 8946450 http://zaguan.unizar.es/record/121846/files/texto_completo.pdf Versión publicada 2723272 http://zaguan.unizar.es/record/121846/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:121846 articulos driver 2024-11-22-11:57:31 ARTICLE