000012309 001__ 12309
000012309 005__ 20170831220511.0
000012309 037__ $$aTAZ-TFM-2013-806
000012309 041__ $$aeng
000012309 1001_ $$aLópez Guimet, Júlia
000012309 24500 $$aDevelopment of microfluidic devices with 3D collagen gels for traction force microscopy
000012309 260__ $$aZaragoza$$bUniversidad de Zaragoza$$c2013
000012309 506__ $$aby-nc-sa$$bCreative Commons$$c3.0$$uhttp://creativecommons.org/licenses/by-nc-sa/3.0/
000012309 500__ $$aResumen disponible también en castellano.
000012309 520__ $$aIn the past few decades, it has been widely demonstrated that cells constantly exert traction forces on the cells and matrix surrounding them. This behavior is fundamental in physiological and pathological processes such as embryogenesis and metastasis, therefore studying how cells interact with their environment is vitally important to understand these processes. To contribute to this research field, the present master project aims to adapt a given microfluidic device to the traction force microscopy technique, which is currently the most reliable approach for measuring cell forces. It is of great interest to quantify the forces exerted by cells seeded inside this microfluidic device because this specific device allows the deposition of collagen in a 3D arrangement, which resembles better the cell’s physiological environment than traditional 2D cell cultures. Device adaptation consisted in introduction of fluorescent microbeads into the collagen matrix, and subsequent confocal microscopy imaging of the cultured cells. Both stages required optimization of diverse features, for example: bead size and concentration, cell viability and labeling, fluorescence staining complications, etc. After numerous experiments and information search, the aforementioned features were improved, and ultimately, the microfluidic device was successfully adapted to traction force microscopy. Thus, the final assays produced useful data for performing the cell force calculation.
000012309 521__ $$aMáster Universitario en Ingeniería Biomédica
000012309 540__ $$aDerechos regulados por licencia Creative Commons
000012309 6531_ $$atraction force microscopy
000012309 6531_ $$abeads
000012309 6531_ $$amicrospheres
000012309 6531_ $$amicrofluídica
000012309 6531_ $$amicrofluidic device
000012309 6531_ $$acell migration
000012309 6531_ $$acell forces
000012309 6531_ $$acollagen
000012309 700__ $$aGarcía Aznar, José Manuel$$edir.
000012309 700__ $$aMendoza Cantos, Gracia M.$$edir.
000012309 7102_ $$aUniversidad de Zaragoza$$bIngeniería Mecánica$$cMec. de Medios Contínuos y Teor. de Estructuras
000012309 8560_ $$f676843@celes.unizar.es
000012309 8564_ $$s1908827$$uhttps://zaguan.unizar.es/record/12309/files/TAZ-TFM-2013-806.pdf$$yMemoria (eng)$$zMemoria (eng)
000012309 909CO $$ooai:zaguan.unizar.es:12309$$ptrabajos-fin-master$$pdriver
000012309 950__ $$a
000012309 980__ $$aTAZ$$bTFM$$cEINA