000123857 001__ 123857
000123857 005__ 20240319081028.0
000123857 0247_ $$2doi$$a10.3390/ijms232315258
000123857 0248_ $$2sideral$$a132408
000123857 037__ $$aART-2022-132408
000123857 041__ $$aeng
000123857 100__ $$aAviñó, Anna
000123857 245__ $$aDetection of SARS-CoV-2 Virus by Triplex Enhanced Nucleic Acid Detection Assay (TENADA)
000123857 260__ $$c2022
000123857 5060_ $$aAccess copy available to the general public$$fUnrestricted
000123857 5203_ $$aSARS-CoV-2, a positive-strand RNA virus has caused devastating effects. The standard method for COVID diagnosis is based on polymerase chain reaction (PCR). The method needs expensive reagents and equipment and well-trained personnel and takes a few hours to be completed. The search for faster solutions has led to the development of immunological assays based on antibodies that recognize the viral proteins that are faster and do not require any special equipment. Here, we explore an innovative analytical approach based on the sandwich oligonucleotide hybridization which can be adapted to several biosensing devices including thermal lateral flow and electrochemical devices, as well as fluorescent microarrays. Polypurine reverse-Hoogsteen hairpins (PPRHs) oligonucleotides that form high-affinity triplexes with the polypyrimidine target sequences are used for the efficient capture of the viral genome. Then, a second labeled oligonucleotide is used to detect the formation of a trimolecular complex in a similar way to antigen tests. The reached limit of detection is around 0.01 nM (a few femtomoles) without the use of any amplification steps. The triplex enhanced nucleic acid detection assay (TENADA) can be readily adapted for the detection of any pathogen requiring only the knowledge of the pathogen genome sequence.
000123857 536__ $$9info:eu-repo/grantAgreement/ES/CSIC/COV19-041$$9info:eu-repo/grantAgreement/ES/DGA-FEDER/E15-17R$$9info:eu-repo/grantAgreement/ES/IACS/PT20-00112$$9info:eu-repo/grantAgreement/ES/ISCIII/CB16-01/00263
000123857 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000123857 590__ $$a5.6$$b2022
000123857 592__ $$a1.154$$b2022
000123857 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b66 / 285 = 0.232$$c2022$$dQ1$$eT1
000123857 593__ $$aMedicine (miscellaneous)$$c2022$$dQ1
000123857 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b52 / 178 = 0.292$$c2022$$dQ2$$eT1
000123857 593__ $$aPhysical and Theoretical Chemistry$$c2022$$dQ1
000123857 593__ $$aComputer Science Applications$$c2022$$dQ1
000123857 593__ $$aInorganic Chemistry$$c2022$$dQ1
000123857 593__ $$aSpectroscopy$$c2022$$dQ1
000123857 593__ $$aOrganic Chemistry$$c2022$$dQ1
000123857 593__ $$aMolecular Biology$$c2022$$dQ2
000123857 593__ $$aCatalysis$$c2022$$dQ2
000123857 594__ $$a7.8$$b2022
000123857 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000123857 700__ $$aCuestas-Ayllón, Carlos
000123857 700__ $$aGutiérrez-Capitán, Manuel
000123857 700__ $$aVilaplana, Lluisa
000123857 700__ $$0(orcid)0000-0001-6170-4237$$aGrazu, Valeria
000123857 700__ $$aNoé, Véronique
000123857 700__ $$aBalada, Eva
000123857 700__ $$aBaldi, Antonio
000123857 700__ $$aFélix, Alex J.
000123857 700__ $$aAubets, Eva
000123857 700__ $$aValiuska, Simonas
000123857 700__ $$aDomínguez, Arnau
000123857 700__ $$aGargallo, Raimundo
000123857 700__ $$aEritja, Ramon
000123857 700__ $$aMarco, M.-Pilar
000123857 700__ $$aFernández-Sánchez, César
000123857 700__ $$0(orcid)0000-0003-1081-8482$$aMartínez de la Fuente, Jesús
000123857 700__ $$aCiudad, Carlos J.
000123857 773__ $$g23, 23 (2022), 15258 [20 pp.]$$pInt. j. mol. sci.$$tInternational Journal of Molecular Sciences$$x1661-6596
000123857 8564_ $$s4652408$$uhttps://zaguan.unizar.es/record/123857/files/texto_completo.pdf$$yVersión publicada
000123857 8564_ $$s2801205$$uhttps://zaguan.unizar.es/record/123857/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000123857 909CO $$ooai:zaguan.unizar.es:123857$$particulos$$pdriver
000123857 951__ $$a2024-03-18-16:53:48
000123857 980__ $$aARTICLE