124034 20241125101130.0 doi 10.1016/j.jnutbio.2022.109207 sideral 132636 ART-2023-132636 eng Herrera-Marcos, L. V. Universidad de Zaragoza (orcid)0000-0002-4665-9674 Dietary squalene supplementation decreases triglyceride species and modifies phospholipid lipidomic profile in the liver of a porcine model of non-alcoholic steatohepatitis 2023 Access copy available to the general public Unrestricted Squalene is a key minor component of virgin olive oil, the main source of fat in the Mediterranean diet, and had shown to improve the liver metabolism in rabbits and mice. The present research was carried out to find out whether this effect was conserved in a porcine model of hepatic steatohepatitis and to search for the lipidomic changes involved. The current study revealed that a 0.5% squalene supplementation to a steatotic diet for a month led to hepatic accumulation of squalene and decreased triglyceride content as well as area of hepatic lipid droplets without influencing cholesterol content or fiber areas. However, ballooning score was increased and associated with the hepatic squalene content. Of forty hepatic transcripts related to lipid metabolism and hepatic steatosis, only citrate synthase and a non-coding RNA showed decreased expressions. The hepatic lipidome, assessed by liquid chromatography-mass spectrometry in a platform able to analyze 467 lipids, revealed that squalene supplementation increased ceramide, Cer(36:2), and phosphatidylcholine (PC[32:0], PC[33:0] and PC[34:0]) species and decreased cardiolipin, CL(69:5), and triglyceride (TG[54:2], TG[55:0] and TG[55:2]) species. Plasma levels of interleukin 12p40 increased in pigs receiving the squalene diet. The latter also modified plasma lipidome by increasing TG(58:12) and decreasing non-esterified fatty acid (FA 14:0, FA 16:1 and FA 18:0) species without changes in total NEFA levels. Together this shows that squalene-induced changes in hepatic and plasma lipidomic profiles, non-coding RNA and anti-inflammatory interleukin are suggestive of an alleviation of the disease despite the increase in the ballooning score. info:eu-repo/grantAgreement/ES/DGA/B16-20R info:eu-repo/grantAgreement/ES/ISCIII/CB06-03-1012 info:eu-repo/grantAgreement/ES/MICINN AEI/PID2019-104915RB-I00 info:eu-repo/grantAgreement/ES/MINECO/SAF2016-75441-R info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 4.8 2023 NUTRITION & DIETETICS 18 / 114 = 0.158 2023 Q1 T1 BIOCHEMISTRY & MOLECULAR BIOLOGY 67 / 313 = 0.214 2023 Q1 T1 1.136 2023 Biochemistry 2023 Q1 Endocrinology, Diabetes and Metabolism 2023 Q1 Nutrition and Dietetics 2023 Q1 Clinical Biochemistry 2023 Q1 Molecular Biology 2023 Q2 9.5 2023 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Martínez-Beamonte, R. Universidad de Zaragoza (orcid)0000-0002-8100-5596 Arnal, C. Universidad de Zaragoza (orcid)0000-0003-1197-4276 Barranquero, C. (orcid)0000-0002-8442-8041 Puente-Lanzarote, J. J. Universidad de Zaragoza (orcid)0000-0002-7213-7095 Herrero-Continente, T. Lou-Bonafonte, J. M. Universidad de Zaragoza (orcid)0000-0001-9578-6525 Gonzalo-Romeo, G. Universidad de Zaragoza (orcid)0009-0008-6478-7857 Mocciaro, G. Jenkins, B. Surra, J. C. Universidad de Zaragoza (orcid)0000-0002-5841-0462 Rodríguez-Yoldi, M. J. Universidad de Zaragoza (orcid)0000-0002-3595-7668 Burillo, J. C. Lasheras, R. García-Gil, A. (orcid)0000-0002-8275-7191 Güemes, A. Universidad de Zaragoza (orcid)0000-0002-4437-2581 Koulman, A. Osada, J. Universidad de Zaragoza (orcid)0000-0002-8251-8457 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 1012 410 Universidad de Zaragoza Dpto. Farmac.Fisiol.y Med.L.F. Área Fisiología 2008 700 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Producción Animal 1013 090 Universidad de Zaragoza Dpto. Cirugía Área Cirugía 0 X Universidad de Zaragoza Serv.Gral. Apoyo Investigación Serv. Div. Experimenta. Animal 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 1002 050 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Biología Celular 112 (2023), 109207 [12 pp.] J. nutr. biochem. Journal of Nutritional Biochemistry 0955-2863 3417324 http://zaguan.unizar.es/record/124034/files/texto_completo.pdf Versión publicada 2497821 http://zaguan.unizar.es/record/124034/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:124034 articulos driver 2024-11-22-11:58:41 ARTICLE