Current approaches for the recreation of cardiac ischaemic environment in vitro

Paz-Artigas, L. ; Montero-Calle, P. ; Iglesias-García, O. ; Mazo, M. M. ; Ochoa, I. (Universidad de Zaragoza) ; Ciriza, J. (Universidad de Zaragoza)

Resumen: Myocardial ischaemia is one of the leading dead causes worldwide. Although animal experiments have historically provided a wealth of information, animal models are time and money consuming, and they usually miss typical human patient’s characteristics associated with ischemia prevalence, including aging and comorbidities. Generating reliable in vitro models that recapitulate the human cardiac microenvironment during an ischaemic event can boost the development of new drugs and therapeutic strategies, as well as our understanding of the underlying cellular and molecular events, helping the optimization of therapeutic approaches prior to animal and clinical testing. Although several culture systems have emerged for the recreation of cardiac physiology, mimicking the features of an ischaemic heart tissue in vitro is challenging and certain aspects of the disease process remain poorly addressed. Here, current in vitro cardiac culture systems used for modelling cardiac ischaemia, from self-aggregated organoids to scaffold-based constructs and heart-on-chip platforms are described. The advantages of these models to recreate ischaemic hallmarks such as oxygen gradients, pathological alterations of mechanical strength or fibrotic responses are highlighted. The new models represent a step forward to be considered, but unfortunately, we are far away from recapitulating all complexity of the clinical situations.

Idioma: Inglés

DOI: 10.1016/j.ijpharm.2023.122589

Año: 2023

Publicado en: International Journal of Pharmaceutics 632 (2023), 122589 [17 pp.]
ISSN: 0378-5173

Financiación: info:eu-repo/grantAgreement/EC/H2020/778354/EU/Heart On chip based on induced pluripotent Stem cell Technology for personalized Medicine/CISTEM
Financiación: info:eu-repo/grantAgreement/EC/H2020/829010/EU/Advanced and versatile PRInting platform for the next generation of active Microfluidic dEvices/PRIME
Financiación: info:eu-repo/grantAgreement/EC/H2020/874827/EU/Computational biomechanics and bioengineering 3D printing to develop a personalized regenerative biological ventricular assist device to provide lasting functional support to damaged hearts/BRAV3
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/PI19-01350

Tipo y forma: Revisión (Versión definitiva)

Área (Departamento): Area Histología (Dpto. Anatom.Histolog.Humanas)