124065 20241125101131.0 doi 10.1016/j.jcis.2022.11.138 sideral 132462 ART-2023-132462 eng Paesa, Monica Universidad de Zaragoza Elucidating the mechanisms of action of antibiotic-like ionic gold and biogenic gold nanoparticles against bacteria 2023 Access copy available to the general public Unrestricted The antimicrobial action of gold depends on different factors including its oxidation state in the intra- and extracellular medium, the redox potential, its ability to produce reactive oxygen species (ROS), the medium components, the properties of the targeted bacteria wall, its penetration in the bacterial cytosol, the cell membrane potential, and its interaction with intracellular components. We demonstrate that different gold species are able to induce bacterial wall damage as a result of their electrostatic interaction with the cell membrane, the promotion of ROS generation, and the consequent DNA damage. In-depth genomic and proteomic studies on Escherichia coli confirmed the superior toxicity of Au (III) vs Au (I) based on the different molecular mechanisms analyzed including oxidative stress, bacterial energetic metabolism, biosynthetic processes, and cell transport. At equivalent bactericidal doses of Au (III) and Au (I) eukaryotic cells were not as affected as bacteria did, maintaining unaffected cell viability, morphology, and focal adhesions; however, increased ROS generation and disruption in the mitochondrial membrane potential were also observed. Herein, we shed light on the antimicrobial mechanisms of ionic and biogenic gold nanoparticles against bacteria. Under selected conditions antibiotic-like ionic gold can exert a strong antimicrobial activity while being harmless to human cells. info:eu-repo/grantAgreement/ES/ISCIII/MS19-00092 info:eu-repo/grantAgreement/ES/MICINN/CTQ2017-84473-R info:eu-repo/grantAgreement/ES/MICINN/PID2020-113987RB-I00 info:eu-repo/grantAgreement/ES/MICINN/PID2021-127847OB-I00 info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 9.4 2023 CHEMISTRY, PHYSICAL 32 / 178 = 0.18 2023 Q1 T1 1.76 2023 Biomaterials 2023 Q1 Surfaces, Coatings and Films 2023 Q1 Colloid and Surface Chemistry 2023 Q1 Electronic, Optical and Magnetic Materials 2023 Q1 16.1 2023 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Remirez de Ganuza, Cristina Universidad de Zaragoza Alejo, Teresa (orcid)0000-0002-9324-0446 Yus, Cristina Universidad de Zaragoza (orcid)0000-0002-1505-498X Irusta, Silvia Universidad de Zaragoza (orcid)0000-0002-2966-9088 Arruebo, Manuel Universidad de Zaragoza (orcid)0000-0003-3165-0156 Sebastian, Víctor Universidad de Zaragoza (orcid)0000-0002-6873-5244 Mendoza, Gracia (orcid)0000-0003-2293-363X 5005 555 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Ingeniería Química 5005 790 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Tecnologi. Medio Ambiente 633 (2023), 786-799 J. colloid interface sci. Journal of Colloid and Interface Science 0021-9797 3303498 http://zaguan.unizar.es/record/124065/files/texto_completo.pdf Versión publicada 2264329 http://zaguan.unizar.es/record/124065/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:124065 articulos driver 2024-11-22-11:58:58 ARTICLE