000124410 001__ 124410
000124410 005__ 20240705134145.0
000124410 0247_ $$2doi$$a10.1007/s00216-023-04583-0
000124410 0248_ $$2sideral$$a132828
000124410 037__ $$aART-2023-132828
000124410 041__ $$aeng
000124410 100__ $$0(orcid)0000-0002-3906-4576$$aSanz-Vicente, Isabel$$uUniversidad de Zaragoza
000124410 245__ $$aPortable colorimetric enzymatic disposable biosensor for histamine and simultaneous histamine/tyramine determination using a smartphone
000124410 260__ $$c2023
000124410 5060_ $$aAccess copy available to the general public$$fUnrestricted
000124410 5203_ $$aTyramine oxidase (TAO), peroxidase (HRP), and Amplex Red (AR) have been immobilized on cellulose to obtain disposable biosensors for the determination of histamine. During the enzymatic reaction, AR is oxidized and a pink spot is obtained. Using a smartphone and measuring the G (green) color coordinate, histamine can be determined in the presence of other biogenic amines (putrescine and cadaverine) in concentrations ranging from 2·10−5 M to 5·10−4 M with a 7.5·10−6 M limit of detection (LoD). Despite tyramine interference, experimental conditions are provided which allow rapid and simple histamine and simultaneous histamine/tyramine (semi)quantitative determination in mixtures. Finally, tyramine and histamine were determined in a tuna extract with good results (compared to the reference HPLC–MS method). The methodology can also be applied in solution allowing histamine (and simultaneous histamine/tyramine) determination with a lower LoD (1.8·10−7 M) and a similar selectivity.
000124410 536__ $$9info:eu-repo/grantAgreement/ES/DGA/E25-20R$$9info:eu-repo/grantAgreement/ES/MICINN/PDC2021-121224-I00$$9info:eu-repo/grantAgreement/ES/MICINN/PID2019-105408GB-I00
000124410 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000124410 592__ $$a0.686$$b2023
000124410 593__ $$aBiochemistry$$c2023$$dQ2
000124410 593__ $$aAnalytical Chemistry$$c2023$$dQ2
000124410 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000124410 700__ $$aRivero, Irina
000124410 700__ $$aMarcuello, Lucía
000124410 700__ $$aMontano, María Pilar
000124410 700__ $$0(orcid)0000-0002-7902-6005$$ade Marcos, Susana$$uUniversidad de Zaragoza
000124410 700__ $$0(orcid)0000-0002-8973-5104$$aGalbán, Javier$$uUniversidad de Zaragoza
000124410 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica
000124410 773__ $$g415 (2023), 1777–1786$$pAnal. Bioanal. Chem.$$tAnalytical and Bioanalytical Chemistry$$x1618-2642
000124410 8564_ $$s1422306$$uhttps://zaguan.unizar.es/record/124410/files/texto_completo.pdf$$yVersión publicada
000124410 8564_ $$s2490264$$uhttps://zaguan.unizar.es/record/124410/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000124410 909CO $$ooai:zaguan.unizar.es:124410$$particulos$$pdriver
000124410 951__ $$a2024-07-05-12:47:21
000124410 980__ $$aARTICLE