124410 20241125101133.0 doi 10.1007/s00216-023-04583-0 sideral 132828 ART-2023-132828 eng Sanz-Vicente, Isabel Universidad de Zaragoza (orcid)0000-0002-3906-4576 Portable colorimetric enzymatic disposable biosensor for histamine and simultaneous histamine/tyramine determination using a smartphone 2023 Access copy available to the general public Unrestricted Tyramine oxidase (TAO), peroxidase (HRP), and Amplex Red (AR) have been immobilized on cellulose to obtain disposable biosensors for the determination of histamine. During the enzymatic reaction, AR is oxidized and a pink spot is obtained. Using a smartphone and measuring the G (green) color coordinate, histamine can be determined in the presence of other biogenic amines (putrescine and cadaverine) in concentrations ranging from 2·10−5 M to 5·10−4 M with a 7.5·10−6 M limit of detection (LoD). Despite tyramine interference, experimental conditions are provided which allow rapid and simple histamine and simultaneous histamine/tyramine (semi)quantitative determination in mixtures. Finally, tyramine and histamine were determined in a tuna extract with good results (compared to the reference HPLC–MS method). The methodology can also be applied in solution allowing histamine (and simultaneous histamine/tyramine) determination with a lower LoD (1.8·10−7 M) and a similar selectivity. info:eu-repo/grantAgreement/ES/DGA/E25-20R info:eu-repo/grantAgreement/ES/MICINN/PDC2021-121224-I00 info:eu-repo/grantAgreement/ES/MICINN/PID2019-105408GB-I00 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 3.8 2023 CHEMISTRY, ANALYTICAL 26 / 106 = 0.245 2023 Q1 T1 BIOCHEMICAL RESEARCH METHODS 15 / 85 = 0.176 2023 Q1 T1 0.686 2023 Biochemistry 2023 Q2 Analytical Chemistry 2023 Q2 8.0 2023 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Rivero, Irina Marcuello, Lucía Montano, María Pilar de Marcos, Susana Universidad de Zaragoza (orcid)0000-0002-7902-6005 Galbán, Javier Universidad de Zaragoza (orcid)0000-0002-8973-5104 2009 750 Universidad de Zaragoza Dpto. Química Analítica Área Química Analítica 415 (2023), 1777–1786 Anal. Bioanal. Chem. Analytical and Bioanalytical Chemistry 1618-2642 1422306 http://zaguan.unizar.es/record/124410/files/texto_completo.pdf Versión publicada 2490264 http://zaguan.unizar.es/record/124410/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:124410 articulos driver 2024-11-22-11:59:36 ARTICLE