124411 20241125101133.0 doi 10.1021/acs.nanolett.2c03593 sideral 132834 ART-2023-132834 eng Rubio-Ruiz, Belén In Cellulo Bioorthogonal Catalysis by Encapsulated AuPd Nanoalloys: Overcoming Intracellular Deactivation 2023 Access copy available to the general public Unrestricted Bioorthogonal metallocatalysis has opened up a xenobiotic route to perform nonenzymatic catalytic transformations in living settings. Despite their promising features, most metals are deactivated inside cells by a myriad of reactive biomolecules, including biogenic thiols, thereby limiting the catalytic functioning of these abiotic reagents. Here we report the development of cytocompatible alloyed AuPd nanoparticles with the capacity to elicit bioorthogonal depropargylations with high efficiency in biological media. We also show that the intracellular catalytic performance of these nanoalloys is significantly enhanced by protecting them following two different encapsulation methods. Encapsulation in mesoporous silica nanorods resulted in augmented catalyst reactivity, whereas the use of a biodegradable PLGA matrix increased nanoalloy delivery across the cell membrane. The functional potential of encapsulated AuPd was demonstrated by releasing the potent chemotherapy drug paclitaxel inside cancer cells. Nanoalloy encapsulation provides a novel methodology to develop nanoreactors capable of mediating new-to-life reactions in cells info:eu-repo/grantAgreement/EC/H2020/742684/EU/Catalytic Dual-Function Devices Against Cancer/CADENCE This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 742684-CADENCE info:eu-repo/grantAgreement/ES/MCIU/RTI2018-099019-A-I00 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 9.6 2023 CHEMISTRY, PHYSICAL 30 / 178 = 0.169 2023 Q1 T1 MATERIALS SCIENCE, MULTIDISCIPLINARY 56 / 439 = 0.128 2023 Q1 T1 NANOSCIENCE & NANOTECHNOLOGY 24 / 141 = 0.17 2023 Q1 T1 PHYSICS, CONDENSED MATTER 10 / 79 = 0.127 2023 Q1 T1 CHEMISTRY, MULTIDISCIPLINARY 29 / 231 = 0.126 2023 Q1 T1 PHYSICS, APPLIED 18 / 179 = 0.101 2023 Q1 T1 3.411 2023 Bioengineering 2023 Q1 Chemistry (miscellaneous) 2023 Q1 Nanoscience and Nanotechnology 2023 Q1 Materials Science (miscellaneous) 2023 Q1 Mechanical Engineering 2023 Q1 Condensed Matter Physics 2023 Q1 16.8 2023 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pérez-López, Ana M. Uson, Laura (orcid)0000-0002-4678-7465 Ortega-Liebana, M. Carmen Valero, Teresa Arruebo, Manuel Universidad de Zaragoza (orcid)0000-0003-3165-0156 Hueso, Jose L. Universidad de Zaragoza (orcid)0000-0002-4546-4111 Sebastian, Victor Universidad de Zaragoza (orcid)0000-0002-6873-5244 Santamaria, Jesus Universidad de Zaragoza (orcid)0000-0002-8701-9745 Unciti-Broceta, Asier 5005 555 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Ingeniería Química 23, 3 (2023), 804-811 Nano lett. Nano Letters 1530-6984 6774879 http://zaguan.unizar.es/record/124411/files/texto_completo.pdf Versión publicada 3120826 http://zaguan.unizar.es/record/124411/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:124411 articulos driver 2024-11-22-11:59:37 ARTICLE