124435 20241125101136.0 doi 10.1016/j.foodcont.2022.109298 sideral 132848 ART-2023-132848 eng Esteban, M. Detection of butyric spores by different approaches in raw milks from cow, ewe and goat 2023 Access copy available to the general public Unrestricted Clostridium tyrobutyricum is described as the main causative agent of late blowing defect in cheese. Currently, there are no fast methods to detect this microorganism in raw milk, which would allow determining the use of milk for fresh or cured cheese. The technique commonly used is the Most Probable Number, which is laborious and non-specific. In this work, we present the optimization of a real-time PCR-based detection method for C. tyrobutyricum spores in raw milk samples. This novel approach extracts DNA in a semi-automatic system with magnetic beads. The applicability of the developed procedure has been tested in field milk samples from cow, ewe and goat (n = 202), allowing detection of low levels of butyric spores. Raw milk samples were also analyzed by microbiological culture in a selective medium for butyric bacteria, and positive colonies were identified by multiplex PCR and 16S rDNA sequencing. Apart from C. tyrobutyricum, other Clostridium spp. were identified, which should be considered for further development of detection methods. info:eu-repo/grantAgreement/ES/DGA/INNOVA-A1-043-11 info:eu-repo/grantAgreement/ES/MICINN/AGL2013-44130-R info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 5.6 2023 FOOD SCIENCE & TECHNOLOGY 24 / 173 = 0.139 2023 Q1 T1 1.146 2023 Food Science 2023 Q1 Biotechnology 2023 Q1 12.2 2023 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Díaz, C. Navarro, J. P. Pérez, M. D. Universidad de Zaragoza (orcid)0000-0003-2555-8425 Calvo, M. Universidad de Zaragoza (orcid)0000-0001-5373-9210 Mata, L. Galán-Malo, P. Sánchez, L. Universidad de Zaragoza (orcid)0000-0001-5964-823X 2008 780 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Tecnología de Alimentos 143 (2023), 109298 [8 pp.] Food control Food Control 0956-7135 1637696 http://zaguan.unizar.es/record/124435/files/texto_completo.pdf Versión publicada 2533891 http://zaguan.unizar.es/record/124435/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:124435 articulos driver 2024-11-22-12:00:49 ARTICLE