000124449 001__ 124449
000124449 005__ 20240705134211.0
000124449 0247_ $$2doi$$a10.1007/s00216-023-04571-4
000124449 0248_ $$2sideral$$a132820
000124449 037__ $$aART-2023-132820
000124449 041__ $$aeng
000124449 100__ $$0(orcid)0000-0003-1654-3803$$aCamacho-Aguayo, Javier$$uUniversidad de Zaragoza
000124449 245__ $$aEnzymatically mediated fluorescent copper nanocluster generation for tyramine determination
000124449 260__ $$c2023
000124449 5060_ $$aAccess copy available to the general public$$fUnrestricted
000124449 5203_ $$aThis work details the enzymatic generation of fluorescence nanomaterials and the use of this optical signal as the analytical parameter for the quantification of the substrate. More specifically, fluorescent copper nanoclusters have been obtained during the enzymatic reaction of tyramine oxidase and tyramine in the presence of Cu(II); the fluorescence intensity being proportional to the concentration of tyramine. The nanoclusters obtained show fluorescence at 445 nm by being excited at 320 nm and have been characterized by TEM, EDX, and XPS. The formation mechanism has also been studied, suggesting that under the optimal conditions (0.1 M MES buffer and pH = 6), the formation of the nanoclusters is due to the reducing properties of the product of the enzymatic reaction (p-hydroxybenzaldehyde) in MES buffer. The method shows a linear relationship with the concentration of tyramine in the range from 1.0·10−5 to 2.5·10−4 M, a RSD of 3% (n = 5) and a LOD of 6.3·10−6 M. The method has been applied to the determination of tyramine in sausage with good results.
000124449 536__ $$9info:eu-repo/grantAgreement/ES/DGA/E25-20R$$9info:eu-repo/grantAgreement/ES/DGA-FEDER/Construyendo Europa desde Aragón$$9info:eu-repo/grantAgreement/ES/MICINN/PID2019-105408GB-I00
000124449 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000124449 592__ $$a0.686$$b2023
000124449 593__ $$aBiochemistry$$c2023$$dQ2
000124449 593__ $$aAnalytical Chemistry$$c2023$$dQ2
000124449 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000124449 700__ $$0(orcid)0000-0002-7902-6005$$ade Marcos, Susana$$uUniversidad de Zaragoza
000124449 700__ $$aPericás, Marta
000124449 700__ $$0(orcid)0000-0002-8973-5104$$aGalbán, Javier$$uUniversidad de Zaragoza
000124449 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica
000124449 773__ $$g415, 11 (2023), 2037–2044$$pAnal. Bioanal. Chem.$$tAnalytical and Bioanalytical Chemistry$$x1618-2642
000124449 8564_ $$s1455905$$uhttps://zaguan.unizar.es/record/124449/files/texto_completo.pdf$$yVersión publicada
000124449 8564_ $$s2183996$$uhttps://zaguan.unizar.es/record/124449/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000124449 909CO $$ooai:zaguan.unizar.es:124449$$particulos$$pdriver
000124449 951__ $$a2024-07-05-12:51:49
000124449 980__ $$aARTICLE